No conflicts of interest exist for any of the authors of the article.
Main Research Article
Frequent detection of cytomegalovirus and Epstein–Barr virus in cervical secretions from healthy young women
Article first published online: 29 MAR 2013
© 2013 The Authors Acta Obstetricia et Gynecologica Scandinavica © 2013 Nordic Federation of Societies of Obstetrics and Gynecology
Acta Obstetricia et Gynecologica Scandinavica
Volume 92, Issue 6, pages 706–710, June 2013
How to Cite
Frequent detection of cytomegalovirus and Epstein–Barr virus in cervical secretions from healthy young women. Acta Obstet Gynecol Scand 2013; DOI: 10.1111/aogs.12134, , , , , .
- Issue published online: 16 MAY 2013
- Article first published online: 29 MAR 2013
- Accepted manuscript online: 29 MAR 2013 03:11AM EST
- Manuscript Accepted: 26 FEB 2013
- Manuscript Received: 26 AUG 2012
- Welander Foundation
- The Health & Medical Care Committee of the Regional Executive Board
- Epstein–Barr virus;
- herpes simplex virus;
- polymerase chain reaction;
- viral transmission
To investigate asymptomatic shedding from the uterine cervix of five human herpes viruses: cytomegalovirus (CMV), Epstein–Barr virus (EBV), herpes simplex virus (HSV) type 1 and type 2 and varicella zoster virus (VZV), in young women.
A descriptive study.
Sahlgrenska University Hospital.
Three hundred and five young, healthy Swedish women.
Cervical specimens were analyzed for the presence of viral DNA with a quantitative real-time PCR assay.
Main outcome measures
Detection of viral DNA.
Viral DNA was detected in 66 (21.6%) of the cervical samples. The most common findings were CMV DNA, detected in 35 (11.5%), and EBV DNA, found in 32 (10.5%) of the women. HSV-1 DNA was detected in 5 (1.7%) and HSV-2 DNA in 4 (1.4%), but VZV DNA was not found. The estimated DNA level for the detected viruses was similar with a mean DNA quantity of 2.6 log genome equivalents (Geq)/mL for CMV (range 1.7–4.3), 2.5 log Geq/mL for EBV (range 1.7–4.7), 2.4 log Geq/mL for HSV-1 (range 1.7–3.5) and 2.6 log Geq/mL for HSV-2 (range 1.7–4.1). The simultaneous presence of DNA from two or more herpes viruses was detected in eight specimens.
Asymptomatic shedding of CMV and EBV from the uterine cervix was found in one-fifth of young women. In four of the cervical samples; two with EBV, one with CMV, one with HSV-2, high amounts of viral DNA (>4 log Geq/mL) were detected suggesting a greater risk of transmitting the virus perinatally or sexually.