Generation and characterization of a monoclonal antibody that cross-reacts with the envelope protein from the four dengue virus serotypes
Article first published online: 18 JAN 2013
© 2013 APMIS. Published by John Wiley & Sons Ltd
Volume 121, Issue 9, pages 848–858, September 2013
How to Cite
Generation and characterization of a monoclonal antibody that cross-reacts with the envelope protein from the four dengue virus serotypes. APMIS 2013; 121: 848–858., , , , , , , , , .
- Issue published online: 6 AUG 2013
- Article first published online: 18 JAN 2013
- Manuscript Accepted: 27 NOV 2012
- Manuscript Received: 31 OCT 2011
- Consejo Nacional de Ciencia y Tecnología. Grant Number: salud-2009-01-115401
- Dengue virus;
- monoclonal antibody;
- dengue viruse-envelope protein
Dengue viruses (DENVs; serotypes 1–4) are members of the flavivirus family. The envelope protein (E) of DENV has been defined as the principal antigenic target in terms of protection and diagnosis. Antibodies that can reliably detect the E surface glycoprotein are necessary for describing and mapping new DENV epitopes as well as for developing more reliable and inexpensive diagnostic assays. In this study, we describe the production and characterization of a monoclonal antibody (mAb) against a recombinant DENV-2 E protein that recognizes a sequential antigen in both native and recombinant form located in domain II of the E protein of all four DENV serotypes. We confirmed that this mAb, C21, recognizes a sequence located in the fusion peptide. In addition, C21 does not have neutralizing activity against DENV-2 in an in vitro system. Furthermore, the C21 mAb is an ideal candidate for the development of research reagents for studying DENV biology because it cross-reacts with the four dengue serotypes.