Effect of plant phenolic compounds on biofilm formation by Pseudomonas aeruginosa
Article first published online: 18 APR 2013
© 2013 APMIS Published by John Wiley & Sons Ltd
Volume 121, Issue 11, pages 1073–1081, November 2013
How to Cite
Effect of plant phenolic compounds on biofilm formation by Pseudomonas aeruginosa. APMIS 2013; 121: 1073–1081., , , , , .
- Issue published online: 24 OCT 2013
- Article first published online: 18 APR 2013
- Manuscript Accepted: 18 DEC 2012
- Manuscript Received: 12 JUL 2012
- Ministry of Education and Science of the Russian Federation. Grant Number: 8307
- plant phenols;
- Pseudomonas aeruginosa ;
- N-acyl-homoserine lactones;
- Quorum Sensing
In the natural environment, bacteria predominantly exist in matrix-enclosed multicellular communities associated with various surfaces, referred to as biofilms. Bacteria in biofilms are extremely resistant to antibacterial agents thus causing serious problems for antimicrobial therapy. In this study, we showed that different plant phenolic compounds, at concentrations that did not or weakly suppressed bacterial growth, increased the capacity of Pseudomonas aeruginosa PAO1 to form biofilms. Biofilm formation of P. aeruginosa PAO1 was enhanced 3- to 7-fold under the action of vanillin and epicatechin, and 2- to 2.5-fold in the presence of 4-hydroxybenzoic, gallic, cinnamic, sinapic, ferulic, and chlorogenic acids. At higher concentrations, these compounds displayed an inhibiting effect. Similar experiments carried out for comparison with Agrobacterium tumefaciens C58 showed the same pattern. Vanillin, 4-hydroxybenzoic, and gallic acids at concentrations within the range of 40 to 400 μg/mL increased the production of N–3-oxo-dodecanoyl-homoserine lactone in P. aeruginosa PAO1 which suggests a possible relationship between stimulation of biofilm formation and Las Quorum Sensing system of this bacterium. Using biosensors to detect N-acyl-homoserine lactones (AHL), we demonstrated that the plant phenolics studied did not mimic AHLs.