Enteric gram-negative bacilli suppress Candida biofilms on Foley urinary catheters
Version of Record online: 8 MAY 2013
© 2013 APMIS Published by Blackwell Publishing Ltd.
Volume 122, Issue 1, pages 47–58, January 2014
How to Cite
Enteric gram-negative bacilli suppress Candida biofilms on Foley urinary catheters. APMIS 2014; 122: 47–58., , , , , .
- Issue online: 27 DEC 2013
- Version of Record online: 8 MAY 2013
- Manuscript Accepted: 5 MAR 2013
- Manuscript Received: 6 SEP 2012
- Council of the University of Hong Kong. Grant Number: 201109176041
- Candida ;
- artificial urine;
Mixed Candida-bacterial biofilms in urinary catheters are common in hospitalized patients. (i) The aims of this study were to evaluate, quantitatively and qualitatively, the in vitro development of mono- and dual-species biofilms (MSBs and DSBs) of Candida albicans and two enteric gram-negative bacilli (EGNB; Pseudomonas aeruginosa or Escherichia coli) on Foley catheter (FC) discs, (ii) to determine the biofilm growth in tryptic soy broth or glucose supplemented artificial urine (AU) and (iii) to assess the inhibitory effects of EGNB and their lipopolysaccharides (LPS) on Candida biofilm growth. The growth of MSBs and DSBs on FC discs was monitored by cell counts and SEM. The metabolic activity of LPS-treated Candida biofilms was determined by the XTT reduction assay. Candida albicans and EGNB demonstrated significant inter- and intra-species differences in biofilm growth on FC discs (p < 0.01). Pseudomonas aeruginosa suppressed Candida albicans significantly (p < 0.001) in DSBs. Compared with MSBs, DSB of EGNB in glucose supplemented AU demonstrated robust growth. Escherichia coli and its LPS, significantly suppressed Candida biofilm growth, compared with Pseudomonas aeruginosa and its LPS (p < 0.001). Candida albicans and EGNB colonization in FC is significantly increased in AU with glucose, and variably modified by Escherichia coli, Pseudomonas aeruginosa and their corresponding LPS.