Microbiology of folliculitis: a histological study of 39 cases
Article first published online: 8 MAY 2013
© 2013 APMIS Published by Blackwell Publishing Ltd.
Volume 122, Issue 1, pages 25–32, January 2014
How to Cite
Microbiology of folliculitis: a histological study of 39 cases. APMIS 2014; 122: 25–32., , , , , , , , .
- Issue published online: 27 DEC 2013
- Article first published online: 8 MAY 2013
- Manuscript Accepted: 5 MAR 2013
- Manuscript Received: 8 NOV 2012
- in situ hybridization;
- Propionibacterium acnes ;
Folliculitis is a common inflammatory skin syndrome. Several microbial organisms have been put forward as causative agents, but few studies visualized microbes directly in inflamed hair follicles. This retrospective study investigated bacterial and fungal colonization of inflamed hair follicles in patients with clinically diagnosed non-infectious folliculitis. Skin biopsies from 39 folliculitis patients and 27 controls were screened by fluorescence in situ hybridization (FISH) using broad-range bacterial and fungal probes and by immunofluorescence microscopy using a monoclonal antibody towards Gram-positive bacteria. Specific monoclonal and polyclonal antibodies towards Staphylococcus spp. and Propionibacterium acnes were applied for further species identification. Inflamed follicles were associated with bacterial colonization in 10 samples (26%) and fungal colonization in three samples (8%). Staphylococcus spp. were observed in inflamed follicles in seven samples (18%). Two samples were positive for P. acnes, which were identified as either type II or type IB/type III. Both Staphylococcus spp. and P. acnes were seen in macrocolonies/biofilm structures. In conclusion, one-third of patients with clinically diagnosed, non-infectious folliculitis exhibited microbial colonization with predominance of Staphylococcus spp.