Glial fibrillary acidic protein expression during HSV-1 infection in mouse cornea

Authors

  • Ge Zhao,

    1. Shandong Provincial Key Laboratory of Ophthalmology, Shandong Provincial Excellent Innovation Team Program, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China
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  • Hao Chen,

    1. Qingdao University, Qingdao, China
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  • Zicheng Song,

    1. Shandong Provincial Key Laboratory of Ophthalmology, Shandong Provincial Excellent Innovation Team Program, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China
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  • Hongmei Yin,

    1. Shandong Provincial Key Laboratory of Ophthalmology, Shandong Provincial Excellent Innovation Team Program, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China
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  • Yuanyuan Xu,

    1. Shandong Provincial Key Laboratory of Ophthalmology, Shandong Provincial Excellent Innovation Team Program, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China
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  • Min Chen

    Corresponding author
    1. Shandong Provincial Key Laboratory of Ophthalmology, Shandong Provincial Excellent Innovation Team Program, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China
    • Min Chen, Shandong Eye Institute, Qingdao 266071, China. e-mail: chminqd@163.com

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Abstract

This study aimed to investigate the dynamic expression of glial fibrillary acidic protein (GFAP), a common neural factor, in cornea and stromal cells during herpes simplex virus-1 (HSV-1) infection. For each anesthetized BALB/c mouse, the cornea in one eye was inoculated with 1 × 105 plaque forming unit (PFU) of HSV-1, while the contralateral cornea was mock-infected as the control. At different timepoints post-infection, corneal lesion examination by slit-lamp biomicroscopy, corneal histology and HSV-1 DNA detection by real-time PCR were performed to estimate the different stage of HSV-1 infection. The expression of GFAP was examined using real-time PCR, western blotting and immunofluorescence staining. After infected with HSV-1 for 15 days, the mouse corneas began to become clear, the corneal pathology recovered to normal, and HSV-1 DNA almost could not be detected, indicating that HSV-1 was entering a relative quiescent state from the acute infection. The expression of GFAP in HSV-1-infected corneas was comparatively low on day 3, increased slightly on day 7, and further increased thereafter, higher than that in mock-infected corneas on day 15. GFAP detection on the cellular level also indicated that the expression was downregulated in acute HSV-1 infection. GFAP was found to be downregulated in HSV-1 acute infection in cornea and upregulated in late stage, suggesting that GFAP might play some role during HSV-1 infection in cornea.

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