Evaluation of the rapid loop-mediated isothermal amplification assay Illumigene for diagnosis of Clostridium difficile in an outbreak situation

Authors

  • Torbjörn Norén,

    1. Department of Laboratory Medicine, Microbiology, Örebro University Hospital, Örebro, Sweden
    Search for more papers by this author
  • Magnus Unemo,

    1. Department of Laboratory Medicine, Microbiology, Örebro University Hospital, Örebro, Sweden
    Search for more papers by this author
  • Cecilia Magnusson,

    1. Department of Infection Control, Ryhov County Hospital, Jönköping, Sweden
    2. Department of Infectious Diseases, Ryhov County Hospital, Jönköping, Sweden
    Search for more papers by this author
  • Maud Eiserman,

    1. Clinical Microbiology, Department of Laboratory Medicine, Division of Medical Services, Ryhov County Hospital, Jönköping, Sweden
    Search for more papers by this author
  • Andreas Matussek

    Corresponding author
    1. Clinical Microbiology, Department of Laboratory Medicine, Division of Medical Services, Ryhov County Hospital, Jönköping, Sweden
    • Andreas Matussek, Clinical Microbiology, Department of Laboratory Medicine, Ryhov County Hospital, Jönköping 55185, Sweden. e-mail: andreas.matussek@lj.se

    Search for more papers by this author

  • [The copyright line for this article was changed on 13 March 2015 after original online publication.]

Abstract

An outbreak of Clostridium difficile infection (CDI) at Höglandet Hospital Eksjö in southern Sweden in 2011 was mainly due to a multidrug-resistant PCR ribotype 046 (30% of all samples). Diagnostics used routinely was the Vidas CDAB assay, but to control the outbreak the rapid loop-mediated isothermal amplification (LAMP) assay Illumigene was introduced and both techniques were compared to Toxigenic culture (TC) prospectively. The LAMP assay had a superior sensitivity, that is, 98% compared to 79% for the Vidas CDAB assay. Most importantly, the mean turn-around-time from collecting sample to result was reduced from 59 h to 2 h enabling early isolation of patients and effective hygiene precautions. This may potentially decrease the morbidity and nosocomial transmissions of C. difficile.

Ancillary