[The copyright line for this article was changed on 13 March 2015 after original online publication.]
Evaluation of the rapid loop-mediated isothermal amplification assay Illumigene for diagnosis of Clostridium difficile in an outbreak situation
Article first published online: 12 JUN 2013
© 2013 The Authors. APMIS published by John Wiley & Sons Ltd. on behalf of Scandinavian Societies for Medical Microbiology and Pathology.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Volume 122, Issue 2, pages 155–160, February 2014
How to Cite
Evaluation of the rapid loop-mediated isothermal amplification assay Illumigene for diagnosis of Clostridium difficile in an outbreak situation. APMIS 2014; 122: 155–160., , , , .
- Issue published online: 23 JAN 2014
- Article first published online: 12 JUN 2013
- Manuscript Accepted: 8 APR 2013
- Manuscript Received: 17 DEC 2012
- Clostridium difficile ;
- LAMP ;
- Vidas CDAB
An outbreak of Clostridium difficile infection (CDI) at Höglandet Hospital Eksjö in southern Sweden in 2011 was mainly due to a multidrug-resistant PCR ribotype 046 (30% of all samples). Diagnostics used routinely was the Vidas CDAB assay, but to control the outbreak the rapid loop-mediated isothermal amplification (LAMP) assay Illumigene was introduced and both techniques were compared to Toxigenic culture (TC) prospectively. The LAMP assay had a superior sensitivity, that is, 98% compared to 79% for the Vidas CDAB assay. Most importantly, the mean turn-around-time from collecting sample to result was reduced from 59 h to 2 h enabling early isolation of patients and effective hygiene precautions. This may potentially decrease the morbidity and nosocomial transmissions of C. difficile.