Commentary: detection of infliximab levels and anti-infliximab antibodies

Authors


Clinicians treating inflammatory bowel disease frequently face the dilemma of loss of response to anti-TNF agents.[1] Previous studies have demonstrated that poor clinical outcomes relate to a lack of circulating drug and the secondary development of antibodies to monoclonal antibodies including infliximab (ATI).[2, 3] However, there is variability and a lack of standardisation in existing infliximab drug level and antidrug antibody assays. The nicely written manuscript by Vande Casteele et al.,[4] addresses these concerns by setting up a robust round robin experiment evaluating both serum samples and spiked control samples to compare three different European assays from the Netherlands, Belgium, and France. While the authors conclude that there was a good correlation of infliximab and ATI measurements between the assays, of concern, the commercially available Biomedical Diagnostics (BMD) kit from Paris, France, detected false positive infliximab levels in nearly a fifth of the samples.

This has implications for the recently published retrospective study by Pariente et al. who utilised the BMD kit and concluded in contrast to existing literature and, perhaps incorrectly, that antibodies to infliximab may not predict response to intensification of infliximab therapy in patients with IBD.[5]

With the recent interest in therapeutic monitoring of anti-TNF therapy to optimise clinical outcomes for patients, there has been an explosion in the number and type of assays reported in the medical literature ranging from solid phase to fluid phase assays, different platforms, including radio-immunoassays and enzyme-linked immunosorbent assays, and differences in the antigenic targets e.g. double-antigen vs. antilambda chain assays.[6-11] In the absence of a gold standard assay, most importantly, this article highlights the need for stringent assessment, standardisation and validation of any future assays before they become commercially available and used for clinical decision making.

Acknowledgement

Declaration of personal and funding interests: None.

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