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Silver catfish Rhamdia quelen immersion anaesthesia with essential oil of Aloysia triphylla (L'Hérit) Britton or tricaine methanesulfonate: effect on stress response and antioxidant status

Authors

  • Luciane Tourem Gressler,

    1. Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
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  • Ana Paula Konzen Riffel,

    1. Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
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  • Thaylise Vey Parodi,

    1. Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
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  • Etiane Medianeira Hundertmarck Saccol,

    1. Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
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  • Gessi Koakoski,

    1. Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
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  • Sílvio Teixeira da Costa,

    1. Departamento de Zootecnia do Centro de Educação Superior Norte do Rio Grande do Sul, Universidade Federal de Santa Maria, Palmeira das Missões, RS, Brazil
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  • Maria Amália Pavanato,

    1. Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
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  • Berta Maria Heinzmann,

    1. Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
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  • Bráulio Caron,

    1. Departamento de Agronomia do Centro de Educação Superior Norte do Rio Grande do Sul, Universidade Federal de Santa Maria, Frederico Westphalen, RS, Brazil
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  • Denise Schmidt,

    1. Departamento de Agronomia do Centro de Educação Superior Norte do Rio Grande do Sul, Universidade Federal de Santa Maria, Frederico Westphalen, RS, Brazil
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  • Susana Francisca Llesuy,

    1. Departamento de Química Analítica y Fisicoquímica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
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  • Leonardo José Gil Barcellos,

    1. Curso de Medicina Veterinária, Universidade de Passo Fundo, Campus Universitário do Bairro São José, Passo Fundo, RS, Brazil
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  • Bernardo Baldisserotto

    Corresponding author
    1. Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
    • Correspondence: B Baldisserotto, Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, 97105.900, Santa Maria, RS, Brazil. E-mail: bbaldisserotto@hotmail.com

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Abstract

Responses to anaesthesia with essential oil (EO) of Aloysia triphylla (135 and 180 mg L−1) and tricaine methanesulfonate (MS222) (150 and 300 mg L−1) were assessed in silver catfish. Exposure to the anaesthetics elicited a stress response in the species. In the case of MS222, it was displayed as a release of cortisol into bloodstream, elevation in hematocrit and plasma ion loss. The EO presented cortisol-blocking properties, but increased haematocrit and disturbances of hydromineral balance were observed. Liver antioxidant/oxidant status of EO and MS222-anaesthetized silver catfish was also estimated. The synthetic anaesthetic induced lipoperoxidation, notwithstanding increased catalase contents, whereas the naturally occurring product was capable of preventing the formation of lipid peroxides, possibly due to combined actions of catalase and glutathione-S-transferase. Anaesthetic efficacy was also tested via induction and recovery times. Overall, the promising results obtained for the physiological parameters of the EO-treated fish counterbalanced the slight prolonged induction time observed for 180 mg L−1. As for 135 mg L−1, both induction and recovery times were lengthy; despite that, the EO was able to promote oxidative protection and mitigate stress. None of the MS222 concentrations prompted such responses concomitantly.

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