Visualization of musculature of the larval greater amberjack Seriola dumerili (Risso) using whole-mount immunostaining with special reference to cranial muscles

Authors

  • Susumu Uji,

    Corresponding author
    1. Aquaculture Technology Division, National Research Institute of Aquaculture, Fisheries Research Agency, Watarai, Mie, Japan
    • Correspondence: S Uji, National Research Institute of Aquaculture, Farming Biology Division, Fisheries Research Agency, 422-1 Minamiise, Watarai, Mie 516-0193, Japan. E-mail: uji@fra.affrc.go.jp

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  • Tadahide Kurokawa,

    1. Stock Enhancement and Aquaculture Division, Tohoku National Fisheries Research Institute, Fisheries Research Agency, Shinhama, Shiogama, Miyagi, Japan
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  • Hiroshi Hashimoto,

    1. Shibushi Laboratory, National Research Institute of Aquaculture, Fisheries Research Agency, Natsui, Shibushi, Kagoshima, Japan
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  • Tohru Suzuki,

    1. Laboratory of Bioindustrial Informatics, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi, Japan
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  • Koichi Okuzawa

    1. Aquaculture Technology Division, National Research Institute of Aquaculture, Fisheries Research Agency, Watarai, Mie, Japan
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Abstract

Muscles play important roles in feeding, respiration and swimming during not only adult stage but also larval stage in fish. However, there is no information of the muscle development in larval greater amberjack, Seriola dumerili (Risso). Here, we investigated muscle development in the greater amberjack, focusing primarily on the cranial muscles from 0 days post hatch (dph) to 12 dph using a modified whole-mount immunohistochemical staining method. We found that the muscles required for feeding develop by 3 dph, when the larvae begin to feed. Subsequently, muscle composition in the dorsal branchial arches changes to the adult form between 5 and 8 dph. At 8 dph, all the muscles required for feeding and respiration appear, whereas the dorsal, pelvic and caudal fin muscles required for swimming develop later. This report provides fundamental information on larval greater amberjack muscle development, which will enable the detection of abnormal larval muscles and improve larval rearing techniques by modifying environmental conditions.

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