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Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury

Authors

  • Yusuke SATO,

    1. Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Fukuoka, Japan
    Current affiliation:
    1. Department of Bio-Productive Science, Faculty of Agriculture, Utsunomiya University, Utsunomiya, Tochigi, Japan
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    • Y. Sato, M.-K. Q. Do and T. Suzuki contributed equally to this work.
  • Mai-Khoi Q. DO,

    1. Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Fukuoka, Japan
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    • Y. Sato, M.-K. Q. Do and T. Suzuki contributed equally to this work.
  • Takahiro SUZUKI,

    1. Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Fukuoka, Japan
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    • Y. Sato, M.-K. Q. Do and T. Suzuki contributed equally to this work.
  • Hideaki OHTSUBO,

    1. Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Fukuoka, Japan
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  • Wataru MIZUNOYA,

    1. Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Fukuoka, Japan
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  • Mako NAKAMURA,

    1. Faculty of Agriculture, Kyushu University, Fukuoka, Japan
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  • Mitsuhiro FURUSE,

    1. Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Fukuoka, Japan
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  • Yoshihide IKEUCHI,

    1. Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Fukuoka, Japan
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  • Ryuichi TATSUMI

    Corresponding author
    • Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Fukuoka, Japan
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Correspondence: Ryuichi Tatsumi, Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi, Fukuoka 812-8581, Japan. (Email: rtatsumi@agr.kyushu-u.ac.jp)

Abstract

Regenerative mechanisms that regulate intramuscular motor innervation. including configuration of the neuromuscular connections are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed a heretofore unexplored role of satellite cells as a key source of a secreted neural chemorepellent semaphorin 3A (Sema3A) expression. In order to verify this concept, there is still a critical need to provide direct evidence to show the up-regulation of Sema3A protein in satellite cells in vivo upon muscle injury. The present study employed a Sema3A/MyoD double-immunohistochemical staining for cryo-sections prepared from cardiotoxin injected gastrocnemius muscle of adult mouse lower hind-limb. Results clearly demonstrated that Sema3A expression was up-regulated in myogenic differentiation-positive satellite cells at 4–12 days post-injury period, the time that corresponds to the cell differentiation phase characterized by increasing myogenin messenger RNA expression. This direct proof encourages a possible implication of satellite cells in the spatiotemporal regulation of extracellular Sema3A concentrations, which potentially ensures coordinating a delay in neurite sprouting and re-attachment of motoneuron terminals onto damaged muscle fibers early in muscle regeneration in synchrony with recovery of muscle-fiber integrity.

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