Ahrong Kim and Dong Han Im contributed equally to this work.
Usefulness of anti-phosphohistone H3 immunoreactivity to determine mitotic rate in gastrointestinal stromal tumors
Article first published online: 19 DEC 2012
Copyright © 2012 The Korean Society for Cytopathology, The Korean Society for Legal Medicine, The Korean Society of Oral and Maxillofacial Pathology, The Korean Society of Pathologists, The Korean Society of Toxicological Pathology, The Korean Society of Veterinary Pathology and Wiley Publishing Asia Pty Ltd
Basic and Applied Pathology
Volume 5, Issue 4, pages 91–97, December 2012
How to Cite
Kim, A., Im, D. H., Kim, K., Kim, J. Y., Sol, M. Y., Lee, J. H. and Choi, K. U. (2012), Usefulness of anti-phosphohistone H3 immunoreactivity to determine mitotic rate in gastrointestinal stromal tumors. Basic and Applied Pathology, 5: 91–97. doi: 10.1111/baap.12003
- Issue published online: 19 DEC 2012
- Article first published online: 19 DEC 2012
- Manuscript Accepted: 20 SEP 2012
- Manuscript Received: 16 APR 2012
- gastrointestinal stromal tumors;
Background and aim
The biologic behavior of gastrointestinal stromal tumors (GIST) is a topic of continuing controversy. The assessment of accurate mitotic figures is known to be one of the major indicators for patients with GIST. However, it is not always easy to search for mitotic figures (MFs) and count them accurately on hematoxylin and eosin stained (H&E) slides.
In this study, phopho-histone H3 (PHH3), which is a recently described specific mitosis marker, was immunohistochemically examined and compared to the mitotic count on H&E sections (H&E-MI) and Ki-67 expression (Ki-67 PI). A hundred cases of histologically confirmed GISTs were reviewed based on counting MF on H&E slides. PHH3 positive MFs (PHH3-MI) were counted in the same way, and the Ki-67 PI was calculated for each case.
A strong correlation was found between PHH3-MI and H&E-MI. Recurrence-free survival was correlated with risk category by National Institutes of Health (NIH) consensus criteria (P = 0.017), mucosal invasion (P = 0.005), H&E-MI (P = 0.002), Ki-67 PI (P = 0.005), and PHH3-MI (P = 0.000). None of these factors was an independent prognostic factor.
Among GISTs, PHH3 staining was primarily found to support grading by facilitating mitotic counting, and it might have a prognostic value in GISTs.