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Figure S1. Alignment of bisulfite treated sequences with that of control (untreated sequence). Three types of cytosines (CG, CHG, and CHH) and their fate under bisulfite treatment are highlighted. (A) Alignment of untreated verses diploid. (B) Alignment of untreated with the TD08 fragment showing the methylation-sensitive amplified polymorphism fragment.

Table S1. Summary of bands appearing in different colchitetraploid generations with EcoRI/HpaII

Table S2. Summary of bands appearing in different colchitetraploid generations with EcoRI/MspI

Table S3. Summary of bands appearing in different colchitetraploid generations with amplified fragment length polymorphism

Table S4. Sequence analysis of methylation-sensitive amplified polymorphism fragments

Table S5. Sequence analysis of amplified fragment length polymorphism fragments

Table S6. Transmission of DNA fragments showing variation in diploids (2x) and different colchitetraploid generations (C0, C1, and C2) of Phlox drummondii by methylation-sensitive amplified polymorphism

Table S7. Transmission of DNA fragments showing variation in diploids (2x) and different colchitetraploid generations (C0, C1, and C2) of Phlox drummondii by amplified fragment length polymorphism

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