Funding sources This work was supported by grants to M.W. from the Deutsche Forschungsgemeinschaft (DFG–SFB650), the Investitionsbank Bank Berlin (IBB-Katapult 1) and by the Charité– Universitätsmedizin Berlin. G.H. was supported by the Deutsche Forschungsgemeinschaft.
Association of vitamin D receptor gene polymorphisms with severe atopic dermatitis in adults
Article first published online: 12 MAR 2013
© 2012 The Authors. BJD © 2012 British Association of Dermatologists
British Journal of Dermatology
Volume 168, Issue 4, pages 855–858, April 2013
How to Cite
Heine, G., Hoefer, N., Franke, A., Nöthling, U., Schumann, R.R., Hamann, L. and Worm, M. (2013), Association of vitamin D receptor gene polymorphisms with severe atopic dermatitis in adults. British Journal of Dermatology, 168: 855–858. doi: 10.1111/bjd.12077
Conflicts of interest None declared.G.H. and N.H. contributed equally to this work; L.H. and M.W. share senior authorship of this work.
- Issue published online: 25 MAR 2013
- Article first published online: 12 MAR 2013
- Accepted manuscript online: 3 OCT 2012 10:58AM EST
- Accepted for publication 27 September 2012
Data S1. Method and supplementary references.
Figure S1. Evolutionary conservation of theVDR gene. Analysis of the VDR gene including5′-2000 bp flanking transcriptional start and 3′-1000bp following final exon 9 were compared between human and mouse,rat and zebrafish.
Figure S2. VDR single nucleotidepolymorphism function. CD14+ monocytes of healthy individualshomozygous for the VDR GCT or AAC haplotype (BsmI,ApaI, TaqI order; GCT is frequent in the severeatopic dermatitis group, AAC in controls) were analysed exvivo or following calcitriol stimulation regarding VDR gene expression determined by quantitative polymerase chain reaction.
Table S1. Patient characteristics
Table S2. Primer sequences for VDR genotyping
Table S3. Product sizes of VDR single nucleotide polymorphisms by restriction fragment length polymorphism
Table S4. Haplotype of donors recruited for functional analysis
Table S5. Oligonucleotides used forquantitative reverse transcription–polymerase chainreaction
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