Identification of autoantibodies expressed in acquired aplastic anaemia

Authors

  • Maki Goto,

    1. Division of Laboratory Diagnosis, Sapporo Medical University Hospital, Sapporo, Japan
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    • These authors contributed equally to this study.
  • Kageaki Kuribayashi,

    1. Division of Laboratory Diagnosis, Sapporo Medical University Hospital, Sapporo, Japan
    2. Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan
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    • These authors contributed equally to this study.
  • Yusuke Takahashi,

    1. Division of Laboratory Diagnosis, Sapporo Medical University Hospital, Sapporo, Japan
    2. Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan
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  • Takashi Kondoh,

    1. Division of Laboratory Diagnosis, Sapporo Medical University Hospital, Sapporo, Japan
    2. Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan
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  • Maki Tanaka,

    1. Division of Laboratory Diagnosis, Sapporo Medical University Hospital, Sapporo, Japan
    2. Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan
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  • Daisuke Kobayashi,

    1. Division of Laboratory Diagnosis, Sapporo Medical University Hospital, Sapporo, Japan
    2. Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan
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  • Naoki Watanabe

    Corresponding author
    1. Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan
    • Division of Laboratory Diagnosis, Sapporo Medical University Hospital, Sapporo, Japan
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Correspondence: Naoki Watanabe, Division of Laboratory Diagnosis, Sapporo Medical University Hospital, Department of Clinical Laboratory Medicine, School of Medicine, Sapporo Medical University, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan.

E-mail: watanabn@sapmed.ac.jp

Summary

Acquired aplastic anaemia (aAA) is recognized as an autoimmune disorder; however, the autoantigens and target cells involved remain elusive. Expression of autoantibodies and their target cells were examined using the haematopoietic cell line K562 and bone marrow stromal cell line hTS-5; 43·5% and 21·7% of aAA expressed autoantibody against K562 and hTS-5 cells, respectively. The autoantigens were identified by serological identification of antigens through recombinant cDNA expression cloning. This study indicates that haematopoietic cells are the targets of immune abnormality in aAA. These autoantibodies may be utilized to distinguish patients associated with immune abnormality from bone marrow failure syndrome.

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