MBD5 regulates iron metabolism via methylation-independent genomic targeting of Fth1 through KAT2A in mice
Article first published online: 18 APR 2014
© 2014 John Wiley & Sons Ltd
British Journal of Haematology
Volume 166, Issue 2, pages 279–291, July 2014
How to Cite
Tao, Y., Wu, Q., Guo, X., Zhang, Z., Shen, Y. and Wang, F. (2014), MBD5 regulates iron metabolism via methylation-independent genomic targeting of Fth1 through KAT2A in mice. British Journal of Haematology, 166: 279–291. doi: 10.1111/bjh.12863
- Issue published online: 1 JUL 2014
- Article first published online: 18 APR 2014
- Manuscript Accepted: 25 FEB 2014
- Manuscript Received: 27 DEC 2013
- Ministry of Science and Technology of China. Grant Numbers: 2011CB966200, 2012BAD33B05
- National Natural Science Foundation of China. Grant Numbers: 31030039, 31225013, 31330036
- Distinguished Professorship Programme of Zhejiang University
Data S1. Methods.
Fig S1. Real-time analysis of iron metabolism-related gene expression in the liver of wild-type and Mbd5−/− mice.
Fig S2. Mbd5 expression in the intestine, liver and spleen of Mbd5flox/flox (n = 6, female) and Mbd5villin/villin (n = 7, female).
Fig S3. Liver iron content in Mbd5villin/villin and Mbd5alb/alb mice at 8 weeks of age.
Fig S4. Liver and spleen iron content in Mbd5villin/villin and Mbd5alb/alb mice at 4 months of age.
Fig S5. Body weight of Mbd5flox/flox and Mbd5villin/villin mice during development.
Fig S6. Liver iron content in Mbd5flox/flox (n = 4, two females and two males) and Mbd5nestin/nestin (n = 3, two females and one male) mice at the age of 8 d post birth.
Fig S7. Western blot analysis of the truncated proteins (Mbd5-FL, Mbd5-N and Mbd5-C) which are tranfected in HEK293 cells.
Table SI. Real-time PCR primers.
Table SII. Analysis of DNA motifs in the Fth1 promoter.
Table SIII. CHIP primers for Fth1 promoter H4 acetylation.
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