These authors contributed equally to this work.
Effects of oxidative stress on red blood cell rheology in sickle cell patients
Version of Record online: 23 APR 2014
© 2014 John Wiley & Sons Ltd
British Journal of Haematology
Volume 166, Issue 4, pages 601–606, August 2014
How to Cite
Hierso, R., Waltz, X., Mora, P., Romana, M., Lemonne, N., Connes, P. and Hardy-Dessources, M.-D. (2014), Effects of oxidative stress on red blood cell rheology in sickle cell patients. British Journal of Haematology, 166: 601–606. doi: 10.1111/bjh.12912
- Issue online: 25 JUL 2014
- Version of Record online: 23 APR 2014
- Manuscript Accepted: 20 MAR 2014
- Manuscript Received: 5 FEB 2014
- Regional Council of Guadeloupe
- sickle cell disease;
- oxidative stress;
Sickle cell anaemia (SS) and sickle cell-haemoglobin C disease (SC) patients exhibit severe red blood cell (RBC) rheological alterations involved in the development of several complications. The contribution of oxidative stress in these haemorheological abnormalities is still unknown. We compared RBC reactive oxygen species (ROS) and glutathione (GSH) content, and the haemorheological profile of SS (n = 11), SC (n = 11) and healthy subjects (n = 12) at baseline and after in-vitro treatment with t-butyl hydroperoxide (TBHP). We showed: (i) higher RBC ROS content in SS and SC patients, with the highest level observed in SS patients; (ii) lower RBC GSH content in sickle syndrome patients, especially in SS patients; (iii) TBHP increased RBC ROS production and decreased RBC GSH content in all groups; (iv) TBHP decreased RBC aggregation and increased the strength of RBC aggregates in all groups but the increase in RBC aggregates strength was greater in sickle cell patients; (v) TBHP decreased RBC deformability in the three groups but with a higher magnitude in sickle cell patients. These data suggest that RBCs from sickle cell patients have an exaggerated response to oxidative stress, which is accompanied by a profound abnormal haemorheological profile, with greater alterations in SS than in SC patients.