Construction of a Plasmodium falciparum Rab-interactome identifies CK1 and PKA as Rab-effector kinases in malaria parasites (pages 34–47)
Fathia Ben Rached, Carinne Ndjembo-Ezougou, Syama Chandran, Hana Talabani, Hélène Yera, Vrushali Dandavate, Pierre Bourdoncle, Markus Meissner, Utpal Tatu and Gordon Langsley
Article first published online: 6 DEC 2011 | DOI: 10.1111/boc.201100081
Rabs are important regulators of vesicular traffic via their recruitment of specific effectors. To identify Rab effectors for the human malaria parasite Plasmodium falciparum we made a yeast Ypt-interactome and exploited this to build a parasite Rab-interactome. The kinase PfCK1 was shown to be a specific PfRab5B effector, whereas the catalytic subunit of PfPKA-C an effector of both PfRab5A and PfRab7. The recruitment of PfPKA to PfRab5A+ and PfRab7+ vesicles suggests that the cAMP-dependent kinase potentially plays a role in early and late endosome function in malaria parasites.