Olesoxime protects embryonic cortical neurons from camptothecin intoxication by a mechanism distinct from BDNF
Article first published online: 25 MAR 2013
© 2012 The Authors. British Journal of Pharmacology © 2012 The British Pharmacological Society
British Journal of Pharmacology
Volume 168, Issue 8, pages 1975–1988, April 2013
How to Cite
Gouarné, C., Giraudon-Paoli, M., Seimandi, M., Biscarrat, C., Tardif, G., Pruss, R. M. and Bordet, T. (2013), Olesoxime protects embryonic cortical neurons from camptothecin intoxication by a mechanism distinct from BDNF. British Journal of Pharmacology, 168: 1975–1988. doi: 10.1111/bph.12094
- Issue published online: 25 MAR 2013
- Article first published online: 25 MAR 2013
- Accepted manuscript online: 28 DEC 2012 04:04AM EST
- Manuscript Accepted: 10 DEC 2012
- Manuscript Revised: 28 NOV 2012
- Manuscript Received: 29 AUG 2012
- European Union. Grant Number: HEALTH-F2-2008-223388
- neurotrophic factors;
- neurodegenerative diseases
Background and Purpose
Olesoxime is a small cholesterol–oxime promoting rat embryonic motor neurons survival in the absence of trophic factors. Because olesoxime can substitute for neurotrophic factors in many situations, and to gain further understanding of its mechanism of action, we wondered if it could prevent neuronal death induced by camptothecin (CPT) and compared its effects with those of brain-derived neurotrophic factor (BDNF).
E17 rat embryonic cortical neurons were treated with olesoxime, BDNF or vehicle and intoxicated with CPT. Caspase-dependent and caspase-independent death pathways along with pro-survival pathways activation were explored.
As previously reported for BDNF, olesoxime dose-dependently delayed CPT-induced cell death. Both compounds acted downstream of p53 activation preventing cytochrome c release and caspases activation. When caspase activation was blocked, both olesoxime and BDNF provided additional neuroprotective effect, potentially through the prevention of apoptosis-inducing factor release from mitochondria. While BDNF activates both the PI3K/Akt and the ERK pathway, olesoxime induced only a late activation of the ERK pathways, which did not seem to play a major role in its neuroprotection against CPT. Rather, our results favour preserved mitochondrial membrane integrity by olesoxime.
Conclusions and Implications
Albeit different, olesoxime and BDNF mechanisms for neuroprotection converge to preserve mitochondrial function. These findings emphasize the importance of targeting the mitochondria in the process of neurodegeneration. Importantly olesoxime, by mimicking neurotrophin pro-survival activities without impacting PI3K/Akt and ERK signalling, may have greater therapeutic potential in many diseases where neurotrophins were considered as a therapeutic solution.