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Figure S1 Effect of K channel inhibition with TEA on hypoxia- and adenosine-induced dilatation in pig coronary artery. (A) Concentration-response curves for O2 lowering in coronary arterial segments without endothelium contracted with PGF (10 μM) in the absence and presence of the K channel inhibitor TEA (1 mM and 10 mM) (n = 7, *P = 0.046, ***P < 0.001 by two-way ANOVA). (B) Concentration-response curves for adenosine in coronary arterial segments without endothelium contracted with PGF (10 μM) in the absence and presence of the K channel inhibitor TEA (1 mM and 10 mM) (n = 7, ***P < 0.001 by two-way ANOVA).

Figure S2 Effect of K channel inhibition on hypoxia-induced dilatation in pig coronary artery. Concentration-response curves for O2 lowering in coronary arterial segments without endothelium contracted with PGF (10 μM) in the absence and presence of (A): 4-AP 0.5 mM (n = 12, *P < 0.001 by two-way ANOVA), (B): IbTX 100 nM (n = 6, *P < 0.001 by two-way ANOVA) or (C): glibenclamide 3 μM (n = 7, *P < 0.05 by two-way ANOVA).

Figure S3 Effect of K channel inhibition on hypoxia-induced dilatation in pig coronary artery. Concentration-response curves for O2 lowering in coronary arterial segments without endothelium contracted with PGF (10 μM) in the absence and presence of (A): 4-AP 0.5 mM and IbTX 100 nM (n = 6, *P < 0.01 by two-way ANOVA), (B): glibenclamide 3 μM and 4-AP 0.5 mM (n = 6, *P < 0.001 by two-way ANOVA)

Figure S4 Effect of K channel inhibition on hypoxia-induced dilatation in pig coronary artery. Concentration-response curves for O2 lowering in coronary arterial segments without endothelium contracted with PGF (10 μM) in the absence and presence of XE991 10 μM, 4-AP 0.5 mM and IbTX 100 nM (n = 7–8, ***P < 0.001 by two-way ANOVA).

Figure S5 Effect of KV7.1 channel inhibition on hypoxia- and adenosine-induced dilatation in pig coronary artery. (A) Concentration-response curves for O2 lowering in coronary arterial segments without endothelium contracted with PGF (10 μM) in the absence and presence of the KV7.1 channel inhibitor chromanol 293B (10 μM) (n = 5, P = 0.22 by two-way ANOVA). (B) Concentration-response curves for adenosine in coronary arterial segments without endothelium contracted with PGF (10 μM) in the absence and presence of the KV7.1 channel inhibitor chromanol 293B (10 μM) (n = 5, P = 0.45 by two-way ANOVA).

Figure S6 Detection of KCa1.1 and KV7 channels by immunoblotting. Immunoblot with samples from a normoxic artery in lane 1 and from a hypoxic artery in lane 2 and showing the presence of (A) KCa1.1α located at 100 kDa (B) KCa1.1β located at 30 kDa (C) KV7.4 located at 77 kDa and (D) KV7.5 located at 100 kDa. (E) Incubated with secondary antibody goat anti-rabbit IgG conjugated to HRP.

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