Author contributions: M Ju, TS Scott-Ward, J Liu and P Khuituan are co-first author.
Loop diuretics are open-channel blockers of the cystic fibrosis transmembrane conductance regulator with distinct kinetics
Version of Record online: 10 DEC 2013
© 2013 The British Pharmacological Society
British Journal of Pharmacology
Volume 171, Issue 1, pages 265–278, January 2014
How to Cite
Ju, M., Scott-Ward, T. S., Liu, J., Khuituan, P., Li, H., Cai, Z., Husbands, S. M. and Sheppard, D. N. (2014), Loop diuretics are open-channel blockers of the cystic fibrosis transmembrane conductance regulator with distinct kinetics. British Journal of Pharmacology, 171: 265–278. doi: 10.1111/bph.12458
- Issue online: 10 DEC 2013
- Version of Record online: 10 DEC 2013
- Accepted manuscript online: 10 OCT 2013 05:31AM EST
- Manuscript Accepted: 26 SEP 2013
- Manuscript Revised: 21 SEP 2013
- Manuscript Received: 11 MAY 2013
- Cystic Fibrosis Trust
- University of Bristol
- Overseas Research Students Awards Scheme of Universities UK
- Strategic Scholarships Fellowships Frontier Research Networks
- Office of the Higher Education Commission of Thailand
Figure S1 Shows overlays of the chemical structures of the four loop diuretics studied.
Figure S2 Uses noise analysis to explore the kinetics of CFTR block by loop diuretics.
Figure S3 Investigates whether loop diuretics are allosteric blockers of CFTR by exploring whether changing the intracellular ATP concentration affects the efficacy of channel block.
Figure S4 Examines whether loop diuretic-blocked channels reopen first before closing on washout of loop diuretic, PKA and ATP from the intracellular solution.
Table S1 Effects of loop diuretics on corner frequencies derived from spectral analysis of CFTR Cl− currents.
Table S2 Effects of furosemide and piretanide on the open- and closed-time constants of wild-type CFTR.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.