Artemis-dependent DNA double-strand break formation at stalled replication forks
Article first published online: 15 APR 2013
© 2013 Japanese Cancer Association
Volume 104, Issue 6, pages 703–710, June 2013
How to Cite
(Cancer Sci, doi: 10.1111/cas.12144, 2013)
- Issue published online: 23 MAY 2013
- Article first published online: 15 APR 2013
- Accepted manuscript online: 6 MAR 2013 12:07PM EST
- Manuscript Accepted: 2 MAR 2013
- Manuscript Revised: 26 FEB 2013
- Manuscript Received: 22 JAN 2013
Doc. S1. Supplementary experimental procedure.
Fig. S1. Cell cycle distribution of S-phase synchronized HeLa cells, quantification of Figure 1(b); ATM activation in HeLa cells transfected with siATR, quantification of Fig. 1(b) .
Fig. S2. Magnified image of γH2AX and RPA2 foci and signal intensity chromatographs.
Fig. S3. RPA2 and phosphorylated ATM colocalization after hydroxyurea treatment, analyzed by immunofluorescence.
Fig. S4. Camptothecin (CPT) induces double-strand breaks.
Fig. S5. Cell cycle profiles of shRNA transfected HeLa cells.
Fig. S6. Quantitation of immunofluorescence data shown in Figure 5(b). Magnified image of phospho-DNA-PKcs (T2609) and RPA2 fociand signal intensity chromatographs. Artemis-independent activation of DNA-PKcs analyzed by Western blotting and immunofluorescence.
Fig. S7. Generation of DSBs dependent on DNA-PKcs analyzed by comet assay.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.