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Tying the knot between cytokine and toll-like receptor signaling in gastrointestinal tract cancers


  • Hazel Tye,

    1. Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
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  • Brendan J. Jenkins

    Corresponding author
    • Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
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To whom correspondence should be addressed.

E-mail: brendan.jenkins@monash.edu


Inflammation-associated malignancies of the gastrointestinal tract (GI), including those of the stomach and colon, collectively rank as the highest cause of cancer-related deaths worldwide. It has been well documented that the deregulated activation of the archetypal pro-inflammatory and oncogenic transcription factors nuclear factor-kappa B (NF-κB) and signal transducer and activator of transcription (STAT)3 is a common feature of GI cancers that invariably correlates with poor prognosis. Signal transducer and activator of transcription 3 and NF-κB are key downstream signal transducers of the interleukin (IL)-6 cytokine and toll-like receptor (TLR) families, respectively, and until recently, the potential involvement of these two families in the pathogenesis of cancer has been investigated in isolation. However, there is now emerging evidence of the complex interplay between the IL-6 cytokine and TLR families in GI tract cancers, with a surprising twist in the identification of a non-immune role for specific TLR family members. In this review, we discuss the molecular mechanisms associated with cross-talk between the IL-6 cytokine family/STAT3 signaling network and the TLR family/NF-κB signaling network, and we address the potential benefit of their therapeutic targeting in gastric and colorectal cancers.

Gastrointestinal (GI) tract cancers affect the stomach, bowel (colon, rectum), esophagus, liver and pancreas, and they collectively rank as the highest cause of cancer mortalities in the world.[1] The human GI tract is heavily colonized by microbes, with studies showing that commensal bacteria play an important role in maintaining intestinal homeostasis.[2, 3] Despite this, deregulated immune responses are strongly associated with the development of inflammation-associated carcinogenesis of the GI tract, and classical examples include Helicobacter (H.) pylori-mediated gastritis and cancer of the stomach (gastric cancer: GC),[4] hepatitis and hepatocellular (liver) cancer,[5] and inflammatory bowel disease and colitis-associated colon cancer (CAC).[6] Indeed, the recognition that chronic inflammation can be a precancerous stage common to GI tract cancers has led to an explosion in research over the last decade, with a primary aim being to identify key factors of the host immune system that promote and maintain carcinogenic changes within the GI mucosal epithelium. Accordingly, in this review, we will focus on recent developments to identify such factors, with an emphasis on the complex interplay between two central molecular families of the immune system, the interleukin (IL)-6 cytokine and toll-like receptor (TLR) families, which display potent pro-inflammatory and oncogenic properties in the stomach and colon.

Epidemiology of GI Cancer

Of the GI tract malignancies, GC and colorectal (bowel) cancer (CRC) have the highest worldwide incidence rates, with approximately 2 million people diagnosed annually.[1] The development of both cancers is a multifactorial process with environmental (e.g. diet, cigarette smoke exposure, microbial infection), epigenetic and genetic factors being implicated.[7, 8] With respect to GC, differentiated intestinal-type adenocarcinoma represents the major histopathological type, accounting for 95% of cases in East Asia, Eastern Europe, and Central and South America. Its etiology is linked primarily to elevated mucosal immune responses following colonization by H. pylori,[4] which infects two-thirds of the world's 7 billion people. However, <3% of H. pylori-infected Japanese individuals will develop GC,[4] which raises a key question as to what host-derived genetic factors determine susceptibility to intestinal-type GC. While the identity of such host-derived susceptibility factors remains ill-defined, one candidate is the pro-inflammatory cytokine IL-1β, for which gene polymorphisms linked to its augmented expression correlate with an increased GC risk in European populations.[9] Another candidate that will be discussed in more detail in this review is TLR2; TLR2 gene polymorphisms are associated with heightened H. pylori-associated GC risk,[10] and increased TLR2 gene expression is significantly elevated in GC patients and correlates with reduced GC patient survival.[11]

Although, approximately 10–30% of CRC cases are inherited and associated with mutations in genes belonging to the DNA mismatch-repair system (MutS Homolog 2 [MSH2] and MutL Homolog 1 [MLH1]), as well as the adenomatosis polyposis coli (Apc) tumor-suppressor gene,[8] inflammatory bowel disease is widely acknowledged as the primary precancerous lesion associated with two-thirds of CAC.[8] Indeed, there is now compelling clinical evidence and data from mouse disease models indicating quantitative and/or qualitative differences in the intestinal microbes that influence disease severity.[12-14] Moreover, a seminal report by Arthur et al. recently revealed that intestinal inflammation can promote tumorigenesis by modifying the composition of intestinal commensals, resulting in an overrepresentation of genotoxic microbes.[15]

Involvement of TLRs in GI Cancers

The strong association between the colonization of microbes and inflammation-associated cancers, such as GC and CRC, has led to intense investigations in recent years into the role of microbial-sensing, host-derived factors of the immune system, namely pattern recognition receptors, in the molecular pathogenesis of these GI cancers. In this respect, much attention has focused on the archetypal class of pattern recognition receptors, the TLR family, the members of which act as critical sensors in the immune system to trigger an inflammatory response following their recognition of pathogen-associated molecular patterns displayed on viral, bacterial and fungal microorganisms.[16] To date, 13 TLRs have been identified in mammals, which are expressed either extracellularly or within endosomes of host cells.[16] The best characterized TLR are TLR4, which recognizes bacterial lipopolysaccharide, and TLR2, which binds to a broad range of ligands (e.g. lipopeptides, peptidoglycan) due to heterodimerization with TLR1 and TLR6.[16] In addition, TLR2 is involved in the recognition of H. pylori to initiate a pro-inflammatory response.[17] Ligand engagement of TLR causes activation of two distinct signaling pathways, myeloid differentiation primary response 88 (MyD88)-dependent and -independent,[16, 18] which are necessary for the activation of the nuclear factor-kappa B (NF-κB) transcriptional complex and MAPK cascade to induce an inflammatory response.[19] Considering NF-κB has a clearly defined role in the initiation and progression of many cancers,[20] it is not surprising that the requirement for MyD88 has been demonstrated in various mouse disease models for intestinal tumorigenesis.[21, 22]

A speculative role for TLRs in mediating the pathogenesis of GC and CRC has come from a spate of studies that have identified genetic polymorphisms in TLR that are associated with disease risk (Table 1). In GC, the most well-characterized genetic polymorphisms are TLR2 –196 to –174del and TLR4 Asp299Gly, which display a strong association with H. pylori-associated non-cardia GC risk in Asian and Western populations, respectively.[10, 23] Interestingly, the TLR4 Asp299Gly mutation is commonly seen in patients with advanced CRC. Furthermore, intestinal epithelial cells that stably expressed TLR4 Asp299Gly displayed enhanced tumorigenicity in a mouse xenograft model compared to the WT allele.[24] In further support of these findings, it is of note that TLR2 and TLR4 are commonly overexpressed in human GC and CRC, respectively.[11, 25, 26]

Table 1. TLR family genetic polymorphisms linked to GC and CRC susceptibility
  1. CRC, colorectal (bowel) cancer; GC, gastric cancer; TLR, toll-like receptor.

GC TLR2 –196 to –174 delTLR2 –196 to –174 del/del is associated with increased risk of GC in Brazilian and Japanese populationsTahara et al.[10]; Zeng et al.[73]
TLR4 A896GTLR4 + 896A/G genotype is associated with increased risk of GC in a Brazilian populationDe Oliveira et al.[74]
TLR5 rs5744174TLR5 rs5744174 C carriers is associated with increased risk of GCZeng et al.[73]
CRC TLR2 C597CTLR2 + 597C/C displayed a five-fold decreased risk of CRCPimental-Nunes et al.[75]
TLR4 D299GAssociated with three-fold increased risk of CRC in EuropeansPimental-Nunes et al.[75]
rs11536898TLR4 A/A genotype is associated with a reduced risk of CRCSlattery et al.[76]

More compelling evidence for the role of TLR2 in GC has come from our recent finding that, among the TLR family members, TLR2 is most significantly overexpressed in the gp130F/F knock-in GC mouse model for spontaneous gastric inflammation and tumorigenesis.[11] Furthermore, we uncovered a novel non-immune role for TLR2 in promoting gastric growth independent of inflammation, whereby activation of TLR2 within (epithelial) tumor cells rather than infiltrating inflammatory cells promoted gastric tumor cell proliferation and survival via upregulation of anti-apoptotic genes (e.g. BCL2-related protein A1 [Bcl2a1], baculoviral IAP repeat containing 3 [Birc3] and B-cell CLL/lymphoma 3 [Bcl3]).[11] With respect to CRC, the use of experimentally induced mouse disease models has primarily implicated TLR4 in promoting disease pathogenesis. For instance, upon azoxymethane (AOM)/dextran sodium sulfate (DSS) treatment, Tlr4−/− mice failed to develop CAC,[27] and a combination of studies involving intestinal epithelial-driven TLR4 transgene expression in mice and TLR4-based bone marrow chimeras indicate that TLR4 expression in the epithelial rather than the myeloid compartment promotes AOM/DSS-induced CAC development.[25, 26] Conversely, the development of larger colorectal tumors in AOM/DSS-treated Tlr2−/− mice compared to their WT counterparts suggests TLR2 may have a protective role in CAC, although the cell types involved were not explored.[28] This latter finding contrasts the reported pro-tumorigenic (epithelial-driven) roles of TLR2 in the stomach and TLR4 in the intestine,[11, 25, 26] and it will be worthwhile to elucidate whether anti-tumorigenic TLR2 signaling in the intestine is propagated by the epithelial or non-epithelial (i.e. inflammatory cells) compartment.

In mouse models for TLR/MyD88-driven CRC, a potential confounding issue in assigning the disease-associated cell type(s) is the divergent nature (i.e. carcinogen-induced vs genetic) of disease models employed. As an example, in a comparison of Myd88−/− and ApcMin/+ mice, genetic deletion of Myd88 significantly reduced the colon tumor burden in ApcMin/+ mice, which was associated with increased apoptosis within the tumor epithelium of ApcMin/+.[21] The reduced tumor load likely arose from an altered (MyD88-dependent) gene expression profile in both the epithelial and stromal (i.e. immune cell) compartments, which ultimately impact epithelial cell survival rather than the infiltration of leukocytes, the latter being analogous to the role of TLR2 and MyD88 in the gp130F/F GC model.[11, 29] While these observations and those regarding the role of TLR4 in DSS/AOM-induced CRC imply that epithelial-derived TLR4/MyD88 signaling primarily promotes intestinal tumorigenesis, TLR/MyD88 signaling in myeloid cells has recently been shown to support spontaneous CRC induced upon Cre-mediated Apc allelic loss in the colon.[22] To relate these divergent findings to the complex pathogenesis of human CRC, it is most likely that TLR/MyD88 signaling in both non-immune (epithelial) and immune (myeloid) cell types is required to elicit the initiation and progression of intestinal tumors, a finding which is supported by the oncogenic anti-apoptotic and pro-inflammatory properties of the NF-κB pathway in the intestinal epithelial and myeloid compartments, respectively.[30]

While the identity of TLR ligands in GC and CRC is also currently unknown, it is worth considering that TLRs not only recognize microbial-derived products, but also host-derived products or danger-associated molecular patterns (DAMP).[19] With respect to DAMP, augmented expression of TLR4 non-microbial ligands, such as high mobility group box 1 (HMGB1) and S100 calcium binding protein A9 (S100A9),[31-34] is often observed in CRC patients, and in GC, the TLR2 agonists versican and serum amyloid A (SAA) are frequently overexpressed.[35-38] Taken together these observations suggest the possible involvement of a combination of pathogen-associated molecular patterns and DAMPs in TLR-driven disease pathogenesis, whereby excessive TLR signaling results from the overexpression of TLRs and/or DAMPs, which serve as TLR agonists.

Interleukin (IL)-6 Family Cytokine Signaling and Cancer

Over the last decade, a wealth of mouse disease models and clinical studies have strongly implicated the IL-6 family of cytokines, in particular IL-6 and IL-11, in malignancies of the digestive system.[39, 40] These cytokines bind to their respective α-receptor subunits, resulting in the activation of the common signal-transducing gp130 β-receptor subunit to activate the JAK/signal transducer and activator of transcription (STAT), Ras/MAPK and phosphoinositide-3-kinase (PI3K)/Akt signaling pathways.[41] With respect to the predominant JAK/STAT pathway, tyrosine residues on the cytoplasmic domain of gp130 are phosphorylated by JAK, which facilitates tyrosine phosphorylation of STAT3.[41] STAT3 phosphorylation results in the formation of homodimers or heterodimers with other STAT molecules, predominantly STAT1, to initiate transcription of STAT target genes in the nucleus.[41] Furthermore, activation of STAT3 is tightly regulated by suppressor of cytokine signaling (SOCS) 3, which negatively regulates STAT3 activity by targeting the receptor complex for proteosomal degradation.[41]

Deregulated STAT3 activation is a common feature of numerous epithelial and hematopoietic malignancies,[42] and the direct oncogenic potential of STAT3 in vivo has been demonstrated upon transgenic overexpression of an artificial hyperactive STAT3 mutant in the lung.[43] Accordingly, much attention has focused on understanding the molecular basis of the pro-tumorigenic actions of STAT3. The broad diversity of STAT3's oncogenic actions is evidenced by its ability to be a potent transcriptional inducer of genes involved in cell cycle progression, cell survival,[44] angiogenesis and inflammation,[45] the last of which exemplifies its oncogenic potency in cancers driven by a chronic inflammatory microenvironment.[46, 47] In addition, inhibiting STAT3 signaling in hematopoietic-derived immune cells has also been shown to improve tumor immune surveillance and elicit multi-component anti-tumor immunity.[48]

The gp130/STAT3-Signaling Axis in GI Cancers

In approximately 50% of human GC, STAT3 is over-activated,[11, 42] and its high activation and/or expression status has been shown to correlate with a lower survival rate for GC patients.[49] While the upstream mechanisms leading to increased STAT3 activity in these studies were not reported, a likely candidate is the gp130-activating cytokine IL-11, which is frequently overexpressed in human GC.[39, 50] Definitive proof that the IL-11/STAT3-signaling axis can be pro-tumorigenic in the stomach was provided by the gp130F/F mouse model for intestinal-type GC, which harbors a homozygous phenylalanine knock-in substitution at tyrosine 757 of the gp130 receptor that abrogates SOCS3 binding, thus resulting in STAT3 hyperactivation. Specifically, these mice spontaneously develop gastritis and gastric tumors within 6–8 weeks, which are then suppressed upon genetic reduction of IL-11-dependent STAT3 activation.[39, 45] Further in vivo evidence for the importance of STAT3 in gastric tumorigenesis has recently been reported. This study used conditional knock-out of SOCS3 in GI epithelial cells (T3b-SOCS3 cKO mice), which spontaneously developed STAT3-mediated gastritis and gastric tumors within 8 weeks, indicating that SOCS3 in the gastric compartment is an important negative regulator for STAT3-mediated gastric tumorigenesis.[51]

Similar to GC, STAT3 is hyperactivated in 90% of colorectal tumor biopsies and correlates with poor prognosis.[52] However, unlike the apparent reliance on IL-11/STAT3 signaling for GC,[39] deregulated IL-6 and IL-11-mediated STAT3 hyperactivation both contribute to the pathogenesis of AOM/DSS-induced CAC.[47] With respect to IL-6, trans-signaling is the main mode by which IL-6 promotes CAC,[40, 53] whereby proteolytic cleavage of membrane-bound IL-6 receptor alpha subunit results in the release of soluble IL-6 receptor alpha subunit. Upon interacting with IL-6, IL-6 receptor alpha subunit enables this complex to associate with gp130 to transduce a signal.[54] The importance of IL-6 trans-signaling in CAC was demonstrated in DSS-treated mice, in which administration with the IL-6 trans-signaling, antagonist-soluble gp130Fc resulted in animals with smaller colonic polyps compared to vehicle-treated animals.[53]

Tying the Knot Between Cytokine and TLR Signaling in GI Cancers

Despite the overwhelming evidence for a causal link between TLR and IL-6 cytokine families in GI cancers, the interplay between these two families in the pathogenesis of such inflammation-associated cancers has not been extensively investigated. An early insight into such potential cross-talk between these families was provided by the often coexistent over-activation of STAT3 and NF-κB, the archetypal transcription factors of the IL-6 cytokine and TLR families in GI tumors, respectively.[55] Upon activation in tumor (i.e. epithelial) cells, both transcription factors induce the expression of overlapping genes involved in cell survival/anti-apoptosis and cell cycle progression, while their activation in inflammatory cells within the stroma propagates the chronic inflammatory state that supports cellular transformation.[11, 30, 56] Evidence for such synergistic interactions between these two transcription factors is provided by the observation that non-phosphorylated versions of STAT3 and NF-κB, in response to IL-6, can form a transcriptional complex that regulates a unique set of inflammatory genes not induced individually by tyrosine phosphorylated STAT3.[57]

An early example that both TLR and IL-6 cytokine family members can cooperate to promote oncogenic cellular responses was provided by evidence that the production of IL-6 is a key event in the MyD88-dependent pathogenesis of diethylnitrosamine-induced liver carcinogenesis.[58] Given the well-documented pro-inflammatory role of IL-6 as a downstream effector of TLR-induced inflammation, this finding is not surprising,[55] but it nonetheless sets the stage for further investigations into the co-dependence of these two families in cancer. In this respect, it has recently emerged that IL-6 family cytokines can influence TLR-induced cellular processes in the GI tract. Specifically, we recently reported that in two independent GC mouse models characterized by elevated STAT3 activation, gp130F/F and Gan mice, STAT3 directly induces the transcription of the Tlr2 gene in the gastric epithelium, which upon overexpression promotes proliferation and inhibits apoptosis of gastric epithelial cells (Fig. 1).[11] Moreover, increased gene expression of TLR2 and over-activation of the STAT3 transcription factor correlated with decreased 5-year survival rates in GC patients.[11] Considering the disparity in the role between specific TLRs (e.g. TLR2, TLR4) in GC and CRC, for instance, it will now be of great interest to examine whether such cross-talk exists in GI cancers between STAT3 and these and other TLR family members.

Figure 1.

The STAT3-driven upregulation of TLR2 promotes gastric tumorigenesis by regulating genes involved in cell proliferation and survival. The induction of several TLR2 DAMPs by TLR2 and/or IL-11/STAT3 signaling pathways may lead to excessive TLR2 activation, resulting in the promotion of gastric epithelial cell growth in a positive feedback loop mechanism. BCL2A1, BCL2-related protein A1; DAMP, danger-associated molecular pattern; gp130, glycoprotein 130; IL, interleukin; NF-κB, nuclear factor-kappa B; PI3K, phosphoinositide 3-kinase; SAA, serum amyloid A; STAT, signal transducer and activator of transcription; TLR, toll-like receptor.

Another consideration in cross-talk between TLR and IL-6 cytokine families is the observation that numerous non-microbial TLR agonists (i.e. DAMPs) are regulated by STAT3. For example, in the context of TLR2 and gastric tumorigenesis, the TLR2 agonists serum amyloid A (SAA) and versican are overexpressed in human GC.[38, 59] While versican is upregulated by IL-11-induced STAT3 activation in human GC cells,[37] SAA is a widely acknowledged STAT3-inducible gene of the acute phase response.[60] In a similar vein, the TLR4 ligands HMGB1 and S100A9, which are upregulated in CRC,[31, 33] are also transcriptionally induced by STAT3.[61, 62] In contrast to the reported IL-11/STAT3-driven role of TLR2 signals on gastric epithelial cell growth in GC,[11] it is more likely in CRC that IL-6/STAT3-induced upregulation of S100A9 and HMGB1 in infiltrating leukocytes induces TLR4-mediated inflammatory and proliferative responses to promote tumorigenesis (Fig. 2).

Figure 2.

Over-activation of TLR4 in IEC promotes the recruitment of macrophages and leukocytes to the lamina propria and subsequently results in IL-6-mediated STAT3 activation to facilitate the production of COX2/PGE2 and the TLR4 DAMP HMGB1. The production of COX2/PGE2 by both IEC and innate immune cells encourages IEC proliferation and survival in CAC. Additionally, STAT3-driven upregulation of HMGB1 may augment TLR4-mediated immune and non-immune signaling in CAC. CAC, colitis-associated colon cancer; CCL2, chemokine (C-C motif) ligand 2; COX2, Cyclooxygenase 2; DAMP, danger-associated molecular patterns; gp130R, glycoprotein 130 receptor; HMGB1, high mobility group box 1; IEC, intestinal epithelial cells; IL, interleukin; PGE, prostaglandin E; STAT, signal transducer and activator of transcription; TLR, toll-like receptor.


The worldwide 5-year overall survival rates for advanced GC and CRC patients are generally poor (approximately 25% and 20%, respectively), which is largely the result of both the late detection of these aggressive diseases and limited effectiveness of current treatment options.[8, 63] In GC, the perioperative adjuvant chemotherapy significantly improved the 5-year survival rate of GI cancer patients compared to surgery-only patients (23% vs 36%), but these survival rates remain unacceptably low.[64] These statistics highlight the need for a more comprehensive understanding of the molecular pathogenesis of these malignancies, which is essential to identifying oncogenic gene networks and signaling pathways that serve as both novel biomarkers for early detection and molecular targets for selected therapeutic intervention. A key consideration for the development of new treatments for GI cancers is the heterogeneous nature of these malignancies, which are characterized by molecular and genetic variations in tumors from patients of different ethnicities and geographic locations.[6, 65] Such heterogeneity among GI cancers highlights the need for targeted, or personalized, therapies to improve a patient's overall survival.

The emergence of a causal role for the IL-6 cytokine and TLR families in GI cancers provides an exciting new avenue for their development into bona fide diagnostic and predictive biomarkers, the latter for patient stratification and druggable therapeutic targets. In GC, this is evidenced by the following: (i) the mechanistic link between IL-11/STAT3 signaling and TLR2 expression; (ii) their co-upregulation in human GC as potential disease-associated biomarkers;[11, 66] and (iii) the therapeutic efficacy of suppressing gastric tumor growth in the gp130F/F GC mouse model with the monoclonal antibody blocking antibody (OPN-301) against TLR2,[11] a STAT3 anti-sense oligonucleotide,[39] or the IL-11-mutein peptide IL-11 antagonist.[67] Although STAT3, like many other transcription factors, serves as a highly attractive cancer therapy target, numerous strategies developed thus far to successfully interfere with STAT3 transcriptional activity in preclinical disease models (with the possible exception of decoy oligonucleotides) have been hampered in their clinical translation due to difficulties with stable and specific drug delivery.[68] Given the lack of clinically proven IL-11 inhibitors, the recent completion of a phase I study demonstrating the safety and tolerability of fully humanized anti-TLR2 monoclonal antibody (OPN-305; Opsona Therapeutics, Dublin, Ireland) paves the way for GC to be examined as a potential clinical target indication for anti-TLR2-based adjuvant therapy (Fig. 3).[69]

Figure 3.

Potential targeted therapies for IL-11/STAT3-driven upregulation of TLR2 in GC. BCL2A1, BCL2-related protein A1; GC, gastric cancer; gp130R, glycoprotein 130 receptor; IL, interleukin; STAT, signal transducer and activator of transcription.

While a definitive link between IL-11 and TLR2 has been reported for GC,[11] such a link between IL-6 cytokine and TLR family members has yet to be formally proven in CRC (and other GI cancers for that matter). Nonetheless, the increased individual expression of TLR4 and IL-6 is a common feature of colorectal cancers and is associated with poor prognosis.[70, 71] In the near future, it will be of great interest to assess the co-upregulation of these family members and their downstream signaling pathways in GI cancers. Furthermore, studies have reported the effectiveness of an antibody-mediated blockade of either TLR4 or IL-6 to reduce the formation of colorectal tumors in experimentally induced mouse cancer models.[25, 53] Unlike TLR4, the clinical efficacy of an IL-6 blockade has been demonstrated with the humanized anti-IL-6 receptor monoclonal antibody tocilizumab in rheumatoid arthritis patients.[72] However, such an antagonist has yet to be proven as an effective anticancer agent in clinical trials. With respect to TLR-related biomarkers and therapeutic targets, other potential candidates include ligands/agonists that signal via TLR. For instance, the TLR2/4 agonists HMGB1 and S100A9 have been touted as potential biomarkers for CRC,[31, 33] as they are significantly upregulated in CRC and have been shown to be regulated by STAT3,[61, 62] which is hyperactivated in approximately 90% of colorectal tumor biopsies.[52] In GC, increased serum levels of SAA and HMGB1 are associated with poor prognostic outcomes,[31, 38] and the fact such proteins can be secreted into the bloodstream makes them attractive candidates for GC biomarkers.

In summary, given the relative paucity of effective therapies against GI cancers, there is a high and largely unmet demand to identify novel therapeutic targets. Until now, the therapeutic targeting of TLR and IL-6 cytokine family members in a range of genetic and preclinical cancer and inflammatory mouse disease models has been considered in isolation. While these therapies are generally efficacious, the potential for translation of such approaches to the clinic now needs to be fully and rigorously investigated. Future studies may benefit substantially by employing a combinatorial approach against both families.


The authors kindly thank members from the Centre for Innate Immunity and Infectious Diseases (Monash University, Clayton, Vic., Australia), in particular Rebecca Smith, for fruitful discussion and proofreading of this manuscript. This work was supported by grants from the National Health and Medical Research Council of Australia and Association for International Cancer Research (UK), as well as the Operational Infrastructure Support Program by the Victorian Government of Australia.

Disclosure Statement

The authors have no conflict of interest.