These authors contributed equally to this work.
HOTAIR, a prognostic factor in esophageal squamous cell carcinoma, inhibits WIF-1 expression and activates Wnt pathway
Article first published online: 29 OCT 2013
© 2013 Japanese Cancer Association
Volume 104, Issue 12, pages 1675–1682, December 2013
How to Cite
(Cancer Sci 2013; 104: 1675–1682)
- Issue published online: 6 DEC 2013
- Article first published online: 29 OCT 2013
- Accepted manuscript online: 1 OCT 2013 12:07AM EST
- Manuscript Accepted: 19 SEP 2013
- Manuscript Revised: 9 SEP 2013
- Manuscript Received: 7 JUN 2013
- National Basic Research Program of China. Grant Number: 2011CB504303
- Ministry of Science and Technology of China. Grant Number: 2011ZX09307-001-04
Long non-coding RNAs (LncRNAs) have been recently found to be pervasively transcribed in the genome and critical regulators of the epigenome. HOTAIR, as a well-known LncRNA, has been found to play important roles in several tumors. Herein, the clinical application value and biological functions of HOTAIR were focused and explored in esophageal squamous cell carcinoma (ESCC). It was found that there was a great upregulation of HOTAIR in ESCC compared to their adjacent normal esophageal tissues. Meanwhile, patients with high HOTAIR expression have a significantly poorer prognosis than those with low expression. Moreover, HOTAIR was further validated to promote migration and invasion of ESCC cells in vitro. Then some specific molecules with great significance were investigated after HOTAIR overexpression using microarray and quantitative real time-polymerase chain reaction (qPCR). WIF-1 playing an important role in Wnt/β-catenin signaling pathway was selected and further tested by immunehistochemistry. Generally, inverse correlation between HOTAIR and WIF-1 expression was demonstrated both in ESCC cells and tissues. Mechanistically, HOTAIR directly decreased WIF-1 expression by promoting its histone H3K27 methylation in the promoter region and then activated the Wnt/β-catenin signaling pathway. This newly identified HOTAIR/WIF-1 axis clarified the molecular mechanism of ESCC cell metastasis and represented a novel therapeutic target in patients with ESCC.