Role of plasmacytoid dendritic cells and inducible costimulator-positive regulatory T cells in the immunosuppression microenvironment of gastric cancer

Authors

  • Xiao-Mei Huang,

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
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    • These authors contributed equally to this work.
  • Xiao-Sun Liu,

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
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    • These authors contributed equally to this work.
  • Xian-Ke Lin,

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
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  • Hang Yu,

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
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  • Jian-Yi Sun,

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
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  • Xiao-Kun Liu,

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
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  • Chao Chen,

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
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  • Hai-Long Jin,

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
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  • Ge-Er Zhang,

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
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  • Xiao-Xiao Shi,

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
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  • Qing Zhang,

    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
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  • Ji-Ren Yu

    Corresponding author
    1. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China
    • Ji-Ren Yu, Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, No. 79, Qingchun Road, 310003 Hangzhou, Zhejiang Province, China.

      Tel: +86-0571-87236147; Fax: +86-0571-87072577;

      E-mail: yujr0909@zju.edu.cn

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Abstract

Regulatory T cells (Tregs) and plasmacytoid dendritic cells (pDCs) play important roles in the immune escape of cancer. In this study, we investigated pDCs and pDC-induced inducible costimulator (ICOS)+ Treg populations in peripheral blood from gastric cancer (GC) patients and healthy donors by flow cytometry. The distribution of these cells in carcinoma tissue, peritumor tissue, and normal gastric mucosa was detected by immunohistochemistry. Plasma and tissue concentration of the cytokines such as interleukin-10 and transforming growth factor-β1 were also measured. We found that the numbers of pDCs, Tregs, and ICOS+ Tregs in peripheral blood were increased in GC patients compared with healthy donors. In tissue, Tregs and ICOS+ Tregs were found distributing mainly in carcinoma tissue, whereas pDCs were mainly found in peritumor tissue. Moreover, the Foxp3+ICOS+/Foxp3+ cell ratio in carcinoma and peritumor tissue were higher than that in normal tissue. There were more ICOS+ Tregs in tumor and peritumor tissue of late-stage GC patients. There was a positive correlation between pDCs and ICOS+ Tregs in peripheral blood and peritumor tissue from GC patients. In conclusion, pDCs may play a potential role in recruiting ICOS+ Tregs, and both participate in the immunosuppression microenvironment of GC.

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