These authors contributed equally to this work.
Role of plasmacytoid dendritic cells and inducible costimulator-positive regulatory T cells in the immunosuppression microenvironment of gastric cancer
Article first published online: 4 JAN 2014
© 2013 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Volume 105, Issue 2, pages 150–158, February 2014
How to Cite
Cancer Sci 105 (2014) 150–158
- Issue published online: 10 FEB 2014
- Article first published online: 4 JAN 2014
- Accepted manuscript online: 22 NOV 2013 12:14AM EST
- Manuscript Accepted: 14 NOV 2013
- Manuscript Revised: 12 NOV 2013
- Manuscript Received: 30 JUL 2013
- National Natural Science Foundation of China. Grant Numbers: 81071964/H1617, 81273254/H1006
- Qianjiang Talent Program of Zhejiang Province. Grant Number: 2012R10046
- Ministry of Science and Technology and Province Department. Grant Number: WKJ2011-2-005
- Dendritic cells;
- immune defense suppression;
- regulatory T cells;
- stomach neoplasms;
- tumor escape
Regulatory T cells (Tregs) and plasmacytoid dendritic cells (pDCs) play important roles in the immune escape of cancer. In this study, we investigated pDCs and pDC-induced inducible costimulator (ICOS)+ Treg populations in peripheral blood from gastric cancer (GC) patients and healthy donors by flow cytometry. The distribution of these cells in carcinoma tissue, peritumor tissue, and normal gastric mucosa was detected by immunohistochemistry. Plasma and tissue concentration of the cytokines such as interleukin-10 and transforming growth factor-β1 were also measured. We found that the numbers of pDCs, Tregs, and ICOS+ Tregs in peripheral blood were increased in GC patients compared with healthy donors. In tissue, Tregs and ICOS+ Tregs were found distributing mainly in carcinoma tissue, whereas pDCs were mainly found in peritumor tissue. Moreover, the Foxp3+ICOS+/Foxp3+ cell ratio in carcinoma and peritumor tissue were higher than that in normal tissue. There were more ICOS+ Tregs in tumor and peritumor tissue of late-stage GC patients. There was a positive correlation between pDCs and ICOS+ Tregs in peripheral blood and peritumor tissue from GC patients. In conclusion, pDCs may play a potential role in recruiting ICOS+ Tregs, and both participate in the immunosuppression microenvironment of GC.