These authors contributed equally to this work.
IDH2 and TP53 mutations are correlated with gliomagenesis in a patient with Maffucci syndrome
Article first published online: 6 MAR 2014
© 2013 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Volume 105, Issue 3, pages 359–362, March 2014
How to Cite
Cancer Sci 105 (2014) 359–362
Platform for Drug Discovery, Informatics, and Structural Life Science from the Ministry of Education, Culture,Sports, Science and Technology (MEXT) of Japan.Regional Innovation Strategy Support Program from MEXT. KAKENHI (25462242): Grant-in-Aid for Scientific Research (C) from MEXT.
- Issue published online: 6 MAR 2014
- Article first published online: 6 MAR 2014
- Accepted manuscript online: 17 DEC 2013 09:17AM EST
- Manuscript Accepted: 13 DEC 2013
- Manuscript Revised: 12 NOV 2013
- Manuscript Received: 21 AUG 2013
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
- KAKENHI. Grant Number: 25462242
Data S1. Materials and methods.
Fig. S1. Mutational analysis of TP53. Direct DNA sequencings of TP53 exon5 (a) and exon10 (b) were performed in the astrocytoma tissues.
Fig. S2. Immunohistochemical analyses against anaplastic astrocytoma tissues using MsMab-1. Mutated IDH2-R172S protein expression was determined immunohistochemically in paraffin-embedded tumor specimens. Briefly, 4-μm-thick histologic sections were deparaffinized in xylene and rehydrated. Then, they were autoclaved in citrate buffer (pH 6.0; Dako) for 20 min. Sections were incubated with 5 μg/ml of MsMab-1 (A) or control (B) overnight at 4°C, followed by treatment with an LSAB kit (Dako). Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (DAB; Dako) for 10 min, and counterstained with hematoxylin. Insets show that MsMab-1 stained cytoplasm. Magnification: ×200.
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