cas12337-sup-0003-DataS1.docWord document31K

Data S1. Materials and methods.


Fig. S1. Mutational analysis of TP53. Direct DNA sequencings of TP53 exon5 (a) and exon10 (b) were performed in the astrocytoma tissues.


Fig. S2. Immunohistochemical analyses against anaplastic astrocytoma tissues using MsMab-1. Mutated IDH2-R172S protein expression was determined immunohistochemically in paraffin-embedded tumor specimens. Briefly, 4-μm-thick histologic sections were deparaffinized in xylene and rehydrated. Then, they were autoclaved in citrate buffer (pH 6.0; Dako) for 20 min. Sections were incubated with 5 μg/ml of MsMab-1 (A) or control (B) overnight at 4°C, followed by treatment with an LSAB kit (Dako). Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (DAB; Dako) for 10 min, and counterstained with hematoxylin. Insets show that MsMab-1 stained cytoplasm. Magnification: ×200.

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