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Data S1. Materials and methods.

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Fig. S1. Mutational analysis of TP53. Direct DNA sequencings of TP53 exon5 (a) and exon10 (b) were performed in the astrocytoma tissues.

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Fig. S2. Immunohistochemical analyses against anaplastic astrocytoma tissues using MsMab-1. Mutated IDH2-R172S protein expression was determined immunohistochemically in paraffin-embedded tumor specimens. Briefly, 4-μm-thick histologic sections were deparaffinized in xylene and rehydrated. Then, they were autoclaved in citrate buffer (pH 6.0; Dako) for 20 min. Sections were incubated with 5 μg/ml of MsMab-1 (A) or control (B) overnight at 4°C, followed by treatment with an LSAB kit (Dako). Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (DAB; Dako) for 10 min, and counterstained with hematoxylin. Insets show that MsMab-1 stained cytoplasm. Magnification: ×200.

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