Reoxygenation from chronic hypoxia promotes metastatic processes in pancreatic cancer through the Hedgehog signaling
Version of Record online: 11 FEB 2014
© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Volume 105, Issue 3, pages 324–333, March 2014
How to Cite
Cancer Sci 105 (2014) 324–333
Japan Society for the Promotion of Science Kakenhi (24390303).
- Issue online: 6 MAR 2014
- Version of Record online: 11 FEB 2014
- Accepted manuscript online: 8 JAN 2014 01:45AM EST
- Manuscript Accepted: 5 JAN 2014
- Manuscript Revised: 24 DEC 2013
- Manuscript Received: 12 JUL 2013
- Japan Society for the Promotion of Science Kakenhi. Grant Number: 24390303
|cas12348-sup-0001_FigS1.tif||image/tif||28K||Fig. S1. Proliferation of Ch-H-R cells that had cultured under hypoxia until 6 months.|
|cas12348-sup-0002_FigS2.tif||image/tif||30K||Fig. S2. Invasive ability within 5 days of reoxygenation in reoxygenated Ch-H-R cells.|
|cas12348-sup-0003_FigS3.tif||image/tif||187K||Fig. S3. The analysis method of immunofluorescent staining.|
|cas12348-sup-0004_FigS4.tif||image/tif||246K||Fig. S4. Immunocytochemistry of CA9 or hypoxia-inducible factor-1α (HIF-1α) in wild type cells, Ac-H cells, Ch-H-R cells and reoxygenated Ch-H-R cells derived from SUIT-2.|
|cas12348-sup-0005_FigS5.tif||image/tif||90K||Fig. S5. Protein expression of hypoxia-inducible factor-1α (HIF-1α) and CA9 by western blotting analysis.|
|cas12348-sup-0006_FigS6.tif||image/tif||126K||Fig. S6. Immunocytochemistry of GLI1 in wild type cells, Ac-H cells, Ch-H-R cells and reoxygenated Ch-H-R cells derived from SUIT-2.|
|cas12348-sup-0007_FigS7.tif||image/tif||65K||Fig. S7. Protein expression of GLI1 by western blotting analysis.|
|cas12348-sup-0008_FigS8.tif||image/tif||160K||Fig. S8. Immunocytochemistry of MMP2 and MMP9 in wild type cells, Ac-H cells, Ch-H-R cells and reoxygenated Ch-H-R cells derived from SUIT-2.|
|cas12348-sup-0009_FigS9.tif||image/tif||157K||Fig. S9. Protein expression of MMP2 and MMP9 by western blotting analysis and gelatin zymography.|
|cas12348-sup-0010_FigS10.tif||image/tif||20K||Fig. S10. Knockdown efficiency of Gli1 mRNA expression on transfection with Gli1-siRNA in reoxygenated Ch-H-R cells.|
|cas12348-sup-0011_FigS11.tif||image/tif||31K||Fig. S11. Knockdown efficiency of MMP2 and MMP9 mRNA expression on transfection with MMP2 and MMP9-siRNA in reoxygenated Ch-H-R cells respectively.|
|cas12348-sup-0012_FigS12.tif||image/tif||48K||Fig. S12. Knock down efficiency of Shh and Smo siRNA transfected cells.|
|cas12348-sup-0013_FigS13.tif||image/tif||121K||Fig. S13. Schematic figure of hypothesis of Ch-H-R cells and reoxygenated Ch-H-R cells.|
|cas12348-sup-0014_FigS14.tif||image/tif||26K||Fig. S14. Secretion of vascular endothelial growth factor (VEGF) in wild type cells, Ac-H cells, Ch-H-R cells and reoxygenated Ch-H-R cells.|
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