Peripheral blood mitochondrial DNA content, A10398G polymorphism, and risk of breast cancer in a Han Chinese population

Authors

  • Huangang Jiang,

    1. Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China
    2. Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, China
    3. Hubei Clinical Cancer Study Center, Wuhan University, Wuhan, China
    Search for more papers by this author
    • These authors contributed equally to this work.
  • Hong Zhao,

    1. Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China
    2. Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, China
    3. Hubei Clinical Cancer Study Center, Wuhan University, Wuhan, China
    Search for more papers by this author
    • These authors contributed equally to this work.
  • Hui Xu,

    1. Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China
    Search for more papers by this author
  • Liu Hu,

    1. Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China
    Search for more papers by this author
  • Wenbo Wang,

    1. Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China
    Search for more papers by this author
  • Yuehua Wei,

    1. Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China
    Search for more papers by this author
  • You Wang,

    1. Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China
    Search for more papers by this author
  • Xiaohong Peng,

    1. Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China
    Search for more papers by this author
  • Fuxiang Zhou

    Corresponding author
    1. Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China
    2. Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, China
    3. Hubei Clinical Cancer Study Center, Wuhan University, Wuhan, China
    • Correspondence

      Fu-Xiang Zhou, Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, 169 Donghu Road, Wuchang District, Wuhan, China.

      Tel: +86 027 6781 3155; Fax: +86 027 6781 2889;

      E-mail: happyzhoufx@sina.com

    Search for more papers by this author

  • Funding Information:

    National Natural Science Foundation of China.

Abstract

It has been reported that quantitative alterations and sequence variations of mtDNA are associated with the onset and progression of particular types of tumor. However, the relationship between mtDNA content, certain mtDNA polymorphisms in peripheral blood leukocytes and breast cancer risk remain obscure. This study was undertaken to investigate whether mtDNA content and the A10398G polymorphism in peripheral blood leukocytes could be used as risk predictors for breast cancer in Han Chinese women. Blood samples were obtained from a total of 506 breast cancer patients and 520 matched healthy controls. The mtDNA content was measured by using quantitative real-time PCR assay; A10398G polymorphism was determined by PCR-RFLP assay. There was no statistically significant difference between cases and controls in terms of peripheral blood mtDNA content or A10398G polymorphism. However, further analysis suggested that the risk of breast cancer was associated with decreased mtDNA content in premenopausal women (= 0.001; odds ratio = 0.54; 95% confidence interval, 0.38–0.77), with increased mtDNA content in postmenopausal women (= 0.027; odds ratio = 1.49; 95% confidence interval, 1.05–2.11). In addition, the associations between mtDNA content and several clinicopathological parameters of cases such as age, menopausal status, and number of pregnancies and live births were observed. This case–control study indicated that the peripheral blood mtDNA content might be a potential biomarker to evaluate the risk of breast cancer for selected Chinese women.

Ancillary