MicroRNA-135a acts as a putative tumor suppressor by directly targeting very low density lipoprotein receptor in human gallbladder cancer

Authors

  • Huading Zhou,

    1. Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China
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    • These authors contributed equally to this work.
  • Weijie Guo,

    1. Shanghai Medical College, Fudan University, Shanghai, China
    2. State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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    • These authors contributed equally to this work.
  • Yingjun Zhao,

    1. State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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  • Yifei Wang,

    1. Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China
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  • Ruopeng Zha,

    1. State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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  • Jie Ding,

    1. State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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  • Linhui Liang,

    1. State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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  • Guanghua Yang,

    1. Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China
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  • Zongyou Chen,

    1. Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China
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  • Baojin Ma,

    Corresponding author
    1. Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China
    2. Jing'an District Center Hospital of Shanghai Huashan Hospital Fudan University Jing'an Branch, Shanghai, China
    • Correspondence

      Baojin Ma and Baobing Yin, Department of General Surgery, Huashan Hospital, Fudan University, No.12 Central Wulumuqi Road, Shanghai 200040, China.

      Tel: +86-21-52887333; Fax: +86-21-52888228;

      E-mails: macswq@126.com; yinbaobing@126.com.

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  • Baobing Yin

    Corresponding author
    1. Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China
    2. Jing'an District Center Hospital of Shanghai Huashan Hospital Fudan University Jing'an Branch, Shanghai, China
    • Correspondence

      Baojin Ma and Baobing Yin, Department of General Surgery, Huashan Hospital, Fudan University, No.12 Central Wulumuqi Road, Shanghai 200040, China.

      Tel: +86-21-52887333; Fax: +86-21-52888228;

      E-mails: macswq@126.com; yinbaobing@126.com.

    Search for more papers by this author

  • Funding Information Science and Technology Commission of Shanghai Municipality (11nm0503900). (12nm0501502). (12QA1403100). Shanghai Municipal Commission of Health and Family Planning (XYQ2011042).

Abstract

The precise functions and mechanisms of microRNAs (miR) in gallbladder cancer (GBC) remain elusive. In this study, we found that miR-135a-5p expression is often dampened and correlated with neoplasm histologic grade in GBC. MicroRNA-135a-5p introduction clearly inhibited GBC cell proliferation in vitro and in vivo. Moreover, very low density lipoprotein receptor (VLDLR), which is often upregulated in GBC tissues, was identified as a direct functional target of miR-135a-5p. Furthermore, the p38 MAPK pathway was proven to be involved in miR-135a-VLDLR downstream signaling. Together, these results suggested that the miR-135a–VLDLR–p38 axis may contribute to GBC cell proliferation.

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