SEARCH

SEARCH BY CITATION

Keywords:

  • affinity screening;
  • competitive binding assay;
  • high-throughput screening;
  • peptide microarray;
  • phosphopeptide;
  • protein tyrosine phosphatase;
  • surface plasmon resonance;
  • Yersinia pestis outer protein H

We have developed competitive and direct binding methods to examine small-molecule inhibitors of protein tyrosine phosphatase activity. Focusing on the Yersinia pestis outer protein H, a potent bacterial protein tyrosine phosphatase, we describe how an understanding of the kinetic interactions involving Yersinia pestis outer protein H, peptide substrates, and small-molecule inhibitors of protein tyrosine phosphatase activity can be beneficial for inhibitor screening, and we further translate these results into a microarray assay for high-throughput screening.