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cea12111-sup-0001-FigS1.pdfapplication/PDF30KFigure S1. Effect of the fish oil diet on total Th1 and Th2 cell subsets in mesenteric lymph nodes (MLN) and spleen by flow cytometry. The percentage of CD4+ cells in MLN (diet NS, sensitization NS, interaction NS) and spleen (diet P < 0.05, sensitization NS, interaction P = 0.09 for spleen) were not reduced by the fish oil diet. The percentage of total Th1 cells (CD4+CXCR3+) was reduced by the fish oil diet in spleen of sham mice (diet P < 0.01, sensitization NS, interaction NS). Percentages of Th1 cells in MLN showed a similar tendency (diet P < 0.05, sensitization NS, interaction NS) as well as the percentage of total Th2 cells (CD4+T1/ST2+) in spleen (P < 0.01, sensitization NS, interaction NS) and MLN (diet NS, sensitization NS, interaction NS). Data are presented as means ± SEM, n = 6/group. *P < 0.05 two-way anova followed by Bonferroni's multiple comparison test. NS = not significant.

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