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cea12117-sup-0001-FigureS1.pdfapplication/PDF26KFigure S1. IgE-levels to fish allergens were determined by an ELISA-based method, as exemplarily shown for patients no. 7 and 12. Sera dilutions falling into the linear range of the standard curve are shown. A. A standard curve based on CSA-specific IgE-dilutions was used to convert OD-units into kUA/L. Patient sera no. 7 and no. 12 were calculated to have 22 kUA/L and 27 kUA/L for cod parvalbumin, respectively. B. OD-values obtained by IgE-reactivity to the different fish allergens are shown. Patient sera no. 7 had specific IgE to fish PV and fish gelatin, but not to the new fish allergens. Patient sera no. 12 had specific IgE to fish PV, fish gelatin, enolases and aldolases (except for cod aldolase). PV, parvalbumin.
cea12117-sup-0002-FigureS2.pdfapplication/PDF417KFigure S2. Purified allergens were heated for 1, 5, 10 and 15 min and analyzed by immunoblot. Commercial polyclonal antibodies detected enolase (A) and aldolase (B) from cod, salmon and tuna.
cea12117-sup-0003-FigureS3.pdfapplication/PDF36KFigure S3. Cod enolase eluted as a dimer and cod aldolase as an oligomer from a gel filtration column, as determined by the comparison of the elution volumes (Ve).
cea12117-sup-0004-TableS1-S9.docWord document449K

Table S1. IgE-cluster I, fish-allergic patients with IgE to parvalbumin from cod, salmon and tuna (children, no. 1–19; adults, no. 20–36).

Table S2. IgE-cluster II, fish-allergic patients with IgE to single parvalbumin (children, no. 37–38; adults, no. 39–45).

Table S3. IgE-cluster III, fish-allergic patients without IgE to fish parvalbumin (children, no. 46–50; adults, no. 51–62).

Table S4. Cod enolase peptides matching with enolase 3 of Danio rerio (gi/68086449) sequence after request in the NCBInr database.

Table S5. Salmon enolase peptides matching with enolase 3-2 of Salmo salar (gi/213513724) sequence after request in the NCBInr database.

Table S6. Tuna enolase peptides matching with enolase 3-2 of Salmo salar (gi/213513724) sequence after request in the NCBInr database.

Table S7. Cod aldolase peptides matching with fructose-bisphosphate aldolase A of Ictalurus puntatus (gi/308321646) sequence after request in the NCBInr database.

Table S8. Salmon aldolase peptides matching with fructose-bisphosphate aldolase A of Salmo salar (gi/213511774) sequence after request in the NCBInr database.

Table S9. Tuna aldolase peptides matching with fructose-bisphosphate aldolase A of Salmo salar (gi/223647884) sequence after request in the NCBInr database.

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