Y.O. and A.M. contributed equally to the manuscript.
Highly expressed cytoplasmic FcεRIβ in human mast cells functions as a negative regulator of the FcRγ-mediated cell activation signal
Article first published online: 22 JAN 2014
© 2013 John Wiley & Sons Ltd
Clinical & Experimental Allergy
Volume 44, Issue 2, pages 238–249, February 2014
How to Cite
Clinical & Experimental Allergy, 2014 (44) 238–249., , , , , , , and ,
- Issue published online: 22 JAN 2014
- Article first published online: 22 JAN 2014
- Accepted manuscript online: 1 OCT 2013 09:13AM EST
- Manuscript Accepted: 26 SEP 2013
- Manuscript Revised: 29 AUG 2013
- Manuscript Received: 18 FEB 2013
- Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government. Grant Number: 23591470
- Nihon University Multidisciplinary Research
Figure S1. Viability of human MCs following infection with Ad5/β-gal (Mock: upper panels) or Ad5/FcεRIβ (lower panels) at a multiplicity of infection (MOI) of 30, 100, or 300 in the presence of SCF.
Figure S2. Efficiency of gene transduction into human MCs by a fibre-mutant adenovirus vector.
Figure S3. Up-regulation of immature and mature FcεRIα in human MCs by FcεRIβ transduction.
Figure S4. Multiplicity of infection-dependent inhibition of histamine release by FcεRIβ-transduced MCs (closed circles) at 30 min after FcεRI aggregation.
Figure S5. Comparison of anti-FcεRIβ Ab, 242+, and Ex3+ MCs in sequential sections of giant papillae.
Figure S6. Expression of cytoplasmic FcεRIβ in MCs of the perivascular regions in the skin from a patient with atopic dermatitis.
Movie S1. FcεRIβ (green) immunostaining of a human MCs of the subepithelial region in a giant papilla. Vertical (Z) images were reconstructed by accumulating horizontal plane (X-Y) images. Blue: nucleus staining using DAPI. Original magnification, ×200.
Movie S2. FcεRIβ (green) immunostaining of a human MCs of the perivascular region in a giant papilla. Vertical (Z) images were reconstructed by accumulating horizontal plane (X-Y) images. Blue: nucleus staining using DAPI. Original magnification, ×200.
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