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cea12210-sup-0001-FigS1-S6-MovieS1-S2.pptapplication/ppt11362K

Figure S1. Viability of human MCs following infection with Ad5/β-gal (Mock: upper panels) or Ad5/FcεRIβ (lower panels) at a multiplicity of infection (MOI) of 30, 100, or 300 in the presence of SCF.

Figure S2. Efficiency of gene transduction into human MCs by a fibre-mutant adenovirus vector.

Figure S3. Up-regulation of immature and mature FcεRIα in human MCs by FcεRIβ transduction.

Figure S4. Multiplicity of infection-dependent inhibition of histamine release by FcεRIβ-transduced MCs (closed circles) at 30 min after FcεRI aggregation.

Figure S5. Comparison of anti-FcεRIβ Ab, 242+, and Ex3+ MCs in sequential sections of giant papillae.

Figure S6. Expression of cytoplasmic FcεRIβ in MCs of the perivascular regions in the skin from a patient with atopic dermatitis.

Movie S1. FcεRIβ (green) immunostaining of a human MCs of the subepithelial region in a giant papilla. Vertical (Z) images were reconstructed by accumulating horizontal plane (X-Y) images. Blue: nucleus staining using DAPI. Original magnification, ×200.

Movie S2. FcεRIβ (green) immunostaining of a human MCs of the perivascular region in a giant papilla. Vertical (Z) images were reconstructed by accumulating horizontal plane (X-Y) images. Blue: nucleus staining using DAPI. Original magnification, ×200.

cea12210-sup-0002-Supplemental.docWord document39K 
cea12210-sup-0003-SupplementalLegends.docWord document41K 

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