Clinical & Experimental Allergy

Quantification of circulating house dust mite-specific IL-4- and IL-13-secreting T cells correlates with rhinitis severity in asthmatic children and varies with the seasons

Authors

  • B. Michaud,

    1. Unité 1013, Institut national de la santé et de la recherche médicale, Paris, France
    2. Faculté de Médecine, Université Paris Descartes, Paris, France
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  • R. Gouvis-Echraghi,

    1. Groupe hospitalier Trousseau-la Roche Guyon, Centre de l'Asthme et des Allergies, Université Pierre et Marie Curie, Paris, France
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  • S. Candon,

    1. Unité 1013, Institut national de la santé et de la recherche médicale, Paris, France
    2. Faculté de Médecine, Université Paris Descartes, Paris, France
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  • R. Couderc,

    1. Groupe hospitalier Trousseau-la Roche Guyon, Service de Biochimie et Biologie Moléculaire, Université Pierre et Marie Curie, Paris, France
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  • J. P. Jais,

    1. Faculté de Médecine, Université Paris Descartes, Paris, France
    2. Biostatistique et Informatique Médicale, Hôpital Necker Enfants Malades, Paris, France
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  • J. F. Bach,

    1. Unité 1013, Institut national de la santé et de la recherche médicale, Paris, France
    2. Faculté de Médecine, Université Paris Descartes, Paris, France
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  • L. Chatenoud,

    Corresponding author
    1. Unité 1013, Institut national de la santé et de la recherche médicale, Paris, France
    2. Faculté de Médecine, Université Paris Descartes, Paris, France
    • Correspondence:

      Lucienne Chatenoud, INSERM U1013, Hôpital Necker Enfants-malades, 149 rue de Sèvres, 75015 Paris, France. E-mail: lucienne.chatenoud@inserm.fr and Jocelyne Just, Hôpital Trousseau, 26 avenue du Dr Arnold Netter, 75012 Paris, France. E-mail: jocelyne.just@trs.aphp.fr

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  • J. Just

    Corresponding author
    1. Groupe hospitalier Trousseau-la Roche Guyon, Centre de l'Asthme et des Allergies, Université Pierre et Marie Curie, Paris, France
    • Correspondence:

      Lucienne Chatenoud, INSERM U1013, Hôpital Necker Enfants-malades, 149 rue de Sèvres, 75015 Paris, France. E-mail: lucienne.chatenoud@inserm.fr and Jocelyne Just, Hôpital Trousseau, 26 avenue du Dr Arnold Netter, 75012 Paris, France. E-mail: jocelyne.just@trs.aphp.fr

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Summary

Background

Defining suitable markers to diagnose and monitor allergy and its severity is essential to correctly assign patients for specific immunotherapy. Circulating levels of specific IgE are good markers of sensitization, but not of clinically symptomatic allergy.

Objective

To quantify circulating interleukin (IL)-4- and IL-13-secreting T cells specific for house dust mite (HDM) in children presenting HDM-allergic asthma associated or not with rhinitis and correlate results with clinical symptoms.

Methods

We analysed 26 children with HDM respiratory disease (allergic rhinitis and asthma) together with six children with non-allergic asthma. Peripheral blood mononuclear cells were stimulated with HDM extract in a 24-h ELISpot assay to quantify the number of HDM-specific IL-4- and IL-13-secreting T cells. Asthma severity and control, and rhinitis severity were scored according to the Global Initiative for Asthma (GINA) and the Allergic Rhinitis and its Impact on Asthma (ARIA) Guidelines.

Results

The number of HDM-specific IL-4- and IL-13-secreting T cells was higher in patients with allergic asthma as compared to patients with non-allergic asthma. It varied with the season of blood sampling with two peaks in the fall and early spring. Independently of the season, the number of HDM-specific IL-4-secreting T cells correlated with rhinitis severity (OR = 2; 95% IC:1.1–3.8; P = 0.04).

Conclusions and Clinical relevance

Allergen-specific IL-4- and IL-13-producing T cells were only detected in HDM-allergic asthmatic children (not in patients with non-allergic asthma). Their numbers correlated with clinical severity of allergic rhinitis.

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