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Figure S1. Sequence alignment of the VH of the four Bet v 1 specific IgE (A; B10 and B13, B; B14 and M0418) together with the V, D and J germline gene segments, from which the clones have their origin. CDR1-3 are underlined

Figure S2. Sequence alignment of the VL of the four Bet v 1-specific IgE B10, B13, B14 and M0418. CDR1-3 are underlined

Figure S3. Biacore inhibition assays of scFv clones B13 (A) and B14 (B) to demonstrate differences in epitope recognition

Figure S4. Epitope mapping of human monoclonal IgE M0418 (A), B13 (B) and B14 (C) (as scFv-Fcε fusion proteins) by ELISA against peptides covering the amino acid sequence of Bet v 1.0101

Figure S5. Mapping of epitopes on Bet v 1 defines three of the regions recognized by antibodies

Figure S6. Sequence (A) of 18 isoallergens/isoforms of Bet v 1 and variability (B) in the different residues calculated as described by Wu and Kabat [S4]

Figure S7. The sequence recognized by M0418 (Bet v 1 residues I56 to D69) exemplified in some members of the PR-10 family of proteins. Identical residues are shaded and indicated by a dot

Figure S8. Proposed model of dimeric Bet v 1 [S5]. The epitope targeted by clone M0418 (residues 56-66) is coloured in pink while that targeted by B13 (residues 26-39) is coloured in blue

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