cea12238-sup-0001-FigureS1.epsimage/eps54KFigure S1. B cells (3 × 105) from healthy laboratory donors were stimulated for 72 h with anti-IgG/M (20 µg/mL), LPS (100 ng/mL), CpG (5 µg/mL) or medium. Medium values (~ 1.5%) were subtracted from the stimulated conditions for each individual. IL-10 secretion assay (Miltenyi Biotec) was performed and the percentage of IL-10 producing B cells is represented (n = 3).
cea12238-sup-0002-FigureS2.epsimage/eps108KFigure S2. Intracellular IL-10 expression (a) in CD24lowCD27, CD24+ or CD27+ B cells. (b) The expression of IL-10 in CD24hiCD27+ B cells excluded for CD1dhi and CD24hiCD38hi B cells. The B cells were treated as described in Fig. 2.
cea12238-sup-0003-FigureS3.epsimage/eps99KFigure S3. Co-cultures of B cell receptor- or CpG-stimulated B cells and T cells. B cells were treated as described in Fig. 2, followed by a 6 days 1:1 co-culture with autologous CD4+ T cells (pre-incubated with isotype or anti-IL-10R antibodies; 2 µg/mL) and DerP1 (1 µg/mL). Cells were restimulated to determine intracellular cytokines by flowcytometry. Percentage in IL-10+ CD4+ T cells was calculated. One pair was excluded as the AA patient was not allergic for HDM (= 12).
cea12238-sup-0004-FigureS4.epsimage/eps60KFigure S4. Immunoglobulin production of LPS-primed B cells in co-culture with T cells. Co-cultures were set up as described in Fig. 4. After 6 days, supernatants were harvested and immunoglobulins (IgG1, IgG2, IgG4 and IgE) were measured by immunoglobuline isotyping assay (Bio-Rad-luminex). Levels of medium-primed and LPS-primed B cells are shown.

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