Allergen hybrids – next generation vaccines for Fagales pollen immunotherapy
Article first published online: 22 FEB 2014
© 2013 The Authors. Clinical & Experimental Allergy published by John Wiley & Sons Ltd.
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Clinical & Experimental Allergy
Volume 44, Issue 3, pages 438–449, March 2014
How to Cite
Clinical & Experimental Allergy, 2014 (44) 438–449., , , , , , , , , , , , , , ,
- Issue published online: 22 FEB 2014
- Article first published online: 22 FEB 2014
- Accepted manuscript online: 11 DEC 2013 10:45AM EST
- Manuscript Accepted: 14 NOV 2013
- Manuscript Revised: 7 OCT 2013
- Manuscript Received: 8 MAR 2013
- Christian Doppler Research Association
- Biomay AG, Vienna, Austria
- Austrian National Bank. Grant Number: 12533
- FWF – Austrian Science Fund. Grant Number: L688
- cytokine ELISA assays
|cea12250-sup-0001-figS1.eps||image/eps||1026K||Figure S1. Inhibition ELISA experiments were performed with sera from Austrian Fagales allergic individuals. Sera were pre-incubated with either the same antigen which was used for coating (black columns), FPH (grey columns), or FPH4 (white columns), respectively.|
|cea12250-sup-0002-figS2.eps||image/eps||1631K||Figure S2. Splenocytes from mice (n = 5 per group) prophylactically treated with the respective antigens or sham were re-stimulated with either parental allergen. Cytokine profiles in supernatants were quantified by FlowCytomix assays.|
|cea12250-sup-0003-figS3.eps||image/eps||800K||Figure S3. (a) Eosinophil counts and (b). IL-5 of BAL fluids of prophylactically treated animals (n = 5 per group) were determined by flowcytometry and ELISA, respectively.|
|cea12250-sup-0004-TableS1.docx||Word document||19K||Table S1. Total IgE measured by EIA (enzyme immunoassay) is presented as IU/L, and specific IgE antibodies determined by ImmunoCAP assays (Thermo Fisher) are presented as kUa/L. Skin prick tests (SPTs) were performed with commercially available extracts and evaluated semi-quantitatively (+ – ≥ 3–<4 mm, ++ – ≥ 4–< 5 mm, +++ – ≥ 5–< 6 mm, ++++ – ≥ 6 mm). F, female; M, male; nd, not determined.|
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