cea12294-sup-0001-Figure-S1.pptapplication/ppt91KFigure S1. Comparison of rFel d 1 and hypoallergenic derivatives by circular dichroism. The mean residue molar ellipticities for rFel d 1 and rFel d 1 derivatives (y-axis: degree cm2 dmol−1) were recorded for a range of wavelengths (x-axis).
cea12294-sup-0002-Figure-S2.pdfPDF document165KFigure S2. (a) SDS–PAGE containing MF under non-reducing and reducing conditions. Lanes M and MF represent the molecular weight marker and purified MF which had been stored for 28 months. Molecular weights are indicated on the left margins. (b) Size exclusion (SEC) profile of MF. The positions of the standards (i.e. dextran blue: 2000 kDa; BSA: 66 kDa; cytochrome c: 12.4 kDa) are indicated, elution volumes (mL) are on the x-axis and the y-axis shows the absorbances at 280 nm.
cea12294-sup-0003-Figure-S3.pptapplication/ppt78KFigure S3. Titration of IgG antibodies specific for rFel d 1. Different dilutions (x-axis) of antisera obtained by immunization of rabbits with rFel d 1(▬) or Fel d 1 derivatives (Fel d 1 M: ■; MB: ▲: MC: –; mix of MB + MC: x; MF: ●) were reacted with ELISA plate-bound rFel d 1. OD values corresponding to bound antibodies are shown on the y-axis. PI: Pre-immune serum.
cea12294-sup-0004-Table-S1.xlsapplication/msexcel19KTable S1. Demographic, clinical and serological characteristics of cat-allergic patients.
cea12294-sup-0005-Table-S2.xlsapplication/msexcel17KTable S2. Characteristics of Fel d 1-derived peptides which were used as building blocks for the derivatives.
cea12294-sup-0006-Table-S3.xlsapplication/msexcel42KTable S3. IgE reactivity of sera from patients with cat allergy to Fel d 1, Feld 1-derived peptides and mosaic derivatives.
cea12294-sup-0007-Table-S4.xlsapplication/msexcel20KTable S4. Percentage inhibition of IgE binding to rFel d 1 after pre-adsorption of patients' sera with rFel d 1, hypoallergenic Fel d 1 derivatives or rPhl p 1.
cea12294-sup-0008-Table-S5.xlsapplication/msexcel26KTable S5. T cell proliferative responses in cat-allergic patients.
cea12294-sup-0009-Table-S6.xlsapplication/msexcel24KTable S6. Inhibition of allergic patients' IgE reactivity to Fel d 1 by IgG antibodies.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.