Myeloid dendritic cells type 2 after allergen inhalation in asthmatic subjects
Article first published online: 23 JUN 2014
© 2014 John Wiley & Sons Ltd
Clinical & Experimental Allergy
Volume 44, Issue 7, pages 921–929, July 2014
How to Cite
Clinical & Experimental Allergy, 2014 (44) 921–929., , , and ,
- Issue published online: 23 JUN 2014
- Article first published online: 23 JUN 2014
- Accepted manuscript online: 27 FEB 2014 12:10PM EST
- Manuscript Accepted: 3 FEB 2014
- Manuscript Revised: 6 JAN 2014
- Manuscript Received: 14 NOV 2013
- Canadian Institutes of Health Research. Grant Number: # 150887
- allergen challenge;
- allergic asthma;
- induced sputum;
- inflammatory cells;
Dendritic cells (DCs) are professional antigen-presenting cells that mediate the response to inhaled allergen. A major division in DC ontogeny exists between myeloid DCs (mDCs) and plasmacytoid DCs (pDCs). A subtype of mDC expressing thrombomodulin, termed myeloid DCs type 2 (mDC2s), has been identified in both the circulation and lung and has recently been suggested to have a role in allergic asthma.
To investigate changes in circulating and sputum mDC2s after allergen inhalation in subjects with asthma.
Peripheral blood and induced sputum were obtained before and 3, 7, and 24 h after inhalation of diluent and allergen from allergic asthmatic subjects who develop both allergen-induced early- and late-phase responses. mDC2s were measured by flow cytometry. Soluble BDCA-3 (thrombomodulin) was measured in sputum by ELISA.
The number of sputum mDC2s significantly increased 24 h after allergen challenge compared with diluent. The expression of BDCA-3 on sputum mDCs also increased, albeit non-significantly, at 7 and 24 h after allergen. Soluble BDCA-3 in sputum and the number of circulating mDC2s were not different between allergen and diluent.
Conclusions and clinical relevance
Myeloid DCs type 2 (mDC2s) increase in the sputum of subjects with asthma after allergen challenge, suggesting this subtype of mDC is involved in the regulation of allergen responses in the lung.