Increased local IgE production induced by common aeroallergens and phenotypic alteration of mast cells in Chinese eosinophilic, but not non-eosinophilic, chronic rhinosinusitis with nasal polyps
Article first published online: 16 APR 2014
© 2014 John Wiley & Sons Ltd
Clinical & Experimental Allergy
Volume 44, Issue 5, pages 690–700, May 2014
How to Cite
Clinical & Experimental Allergy,2014 (44) 690–700., , , , , , , , and ,
- Issue published online: 16 APR 2014
- Article first published online: 16 APR 2014
- Accepted manuscript online: 6 MAR 2014 02:21AM EST
- Manuscript Revised: 24 FEB 2014
- Manuscript Accepted: 24 FEB 2014
- Manuscript Received: 20 NOV 2013
- National Natural Science Foundation of China (NSFC). Grant Numbers: 81325006, 81020108018, 81200733
- Ministry of Health of China. Grant Number: 201202005
- NIH. Grant Numbers: R01HL078860, R37HL068546
- chronic rhinosinusitis;
- immunoglobulin E;
- mast cell;
- nasal polyps
Eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps (CRSwNP) display distinct patterns of inflammation. However, the pathogenic mechanisms underlying the heterogeneity of CRSwNP need further investigation.
To investigate local immunoglobulin E (IgE) production and phenotype of mast cells in eosinophilic and non-eosinophilic CRSwNP in Chinese.
Total and specific IgE levels were analysed by means of the ImmunoCAP system. The molecular steps involved in class-switch recombination to IgE were investigated using RT-PCR assays. Mast cell phenotypes, IgE- and high-affinity IgE receptor (FcεRI)-positive cells, and allergen binding to specific IgE in sinonasal mucosa were determined by means of immunohistochemistry.
Compared with controls and non-eosinophilic CRSwNP, local total IgE levels were increased, and local specific IgE to common aeroallergens was more frequently found, in Chinese eosinophilic CRSwNP independent of atopy and without significant association with Staphylococcus aureus enterotoxins. The ε germline gene transcript was also more frequently detected in eosinophilic CRSwNP. The number of IgE- and FcεRI-positive cells was increased in eosinophilic CRSwNP. Most IgE- and FcεRI-positive cells were mast cells. Dust mite antigens could bind to IgE on mast cells in situ. The number of mast cells positive for both tryptase and chymase and activated mast cells was increased in eosinophilic CRSwNP and the number of activated mast cells positively correlated with local IgE level, eotaxin-1 level, and eosinophil count in CRSwNP.
Conclusions and Clinical Relevance
The local IgE induced by common aeroallergens may mediate mast cell activation and contribute to subsequent eosinophilic inflammation in Chinese CRSwNP. This study offers a rationale for considering intervention strategies designed to target ‘local allergy’ in eosinophilic CRSwNP.