Conflict of interest: none declared.
Experimental dermatology ● Original article
Protective effect of dexamethasone on fetal rat skin in experimental intrauterine ischaemia/reperfusion injury
Article first published online: 22 MAR 2013
© The Author(s) CED © 2013 British Association of Dermatologists.
Clinical and Experimental Dermatology
Volume 38, Issue 4, pages 396–402, June 2013
How to Cite
Kaptanoglu, A. F., Arca, T., Sargon, M. F. and Kilinc, K. (2013), Protective effect of dexamethasone on fetal rat skin in experimental intrauterine ischaemia/reperfusion injury. Clinical and Experimental Dermatology, 38: 396–402. doi: 10.1111/ced.12019
- Issue published online: 23 APR 2013
- Article first published online: 22 MAR 2013
- Manuscript Accepted: 12 JUL 2012
Perinatal asphyxia is an important cause of injury to fetal tissues such as the brain, heart, liver and gastrointestinal system. Fetal skin has also been shown to be vulnerable to intrauterine injury after intrauterine ischaemia/reperfusion (I/R) injury.
To examine the effect of dexamethasone on fetal skin in intrauterine I/R injury in rats.
The response of rat fetal skin to I/R injury and maternal dexamethasone treatment were assessed by determining thiobarbituric acid reactive substances (TBARS), and myeloperoxidase (MPO) and nitric oxide (NO) metabolites. We also examined the ultrastructural changes of fetal skin. Bilateral utero-ovarian artery clamping was performed to produce ischaemia for 30 min in rats at day 19 of pregnancy, and reperfusion was achieved by removing the clamps for 60 min before fetal tissue was collected. The treatment group was given dexamethasone intraperitoneally 20 min before I/R was performed.
TBARS, MPO and NO all increased significantly in fetal rat skin after I/R injury. Levels of TBARS, MPO and NO were significantly lower in the dexamethasone-treated group than in the I/R-only group. I/R injury produced ultrastructural damage in the epidermis. Oedema and mitochondrial damage were less severe in the dexamethasone-treated group.
Maternal treatment with dexamethasone may have a protective effect on fetal skin in cases of I/R injury.