C.-M. Li and W. Li contributed equally to this work, and should be considered joint first authors.
Experimental dermatology ● Original article
Expression of pigment epithelium-derived factor in human cutaneous appendages
Article first published online: 16 MAY 2013
© 2013 British Association of Dermatologists
Clinical and Experimental Dermatology
Volume 38, Issue 6, pages 652–658, August 2013
How to Cite
Li, C.-M., Li, W., Man, X.-Y., Liu, Z.-G. and Zheng, M. (2013), Expression of pigment epithelium-derived factor in human cutaneous appendages. Clinical and Experimental Dermatology, 38: 652–658. doi: 10.1111/ced.12066
Conflict of interest: none declared.
- Issue published online: 10 JUL 2013
- Article first published online: 16 MAY 2013
- Manuscript Accepted: 22 AUG 2012
- National Natural Science Foundation of China. Grant Numbers: 30972643, 81260238
- Natural Science Foundation of Jiangxi Province. Grant Number: 20122BAB215032
- Chinese Medical Association Foundation. Grant Number: H2013101010
Pigment epithelium-derived factor (PEDF), a 50-kDa glycoprotein and a member of the serine protease inhibitor gene family, is well known as a potent endogenous inhibitor of angiogenesis. However, the expression of PEDF in human cutaneous appendages has not yet been determined.
To investigate the expression of PEDF in human cutaneous appendages.
Immunohistochemical staining was used to detect the expression of PEDF in human cutaneous appendages. Reverse transcriptase PCR, western blotting and indirect immunofluorescence were used to determine the mRNA and protein expression of PEDF on cells of the outer root sheath (ORS). A wound-healing assay was used to determine the effect of different concentrations of PEDF on the migration of ORS cells.
PEDF was expressed in the hair follicle (including epidermal matrix, inner root sheath, ORS and fibrous root sheath), sebaceous glands and eccrine sweat glands. Both protein and RNA expression of PEDF was detected, and expression was localized to both cytoplasm and nucleus of ORS cells. Both interleukin (IL)-4 and IL-17 at 25 ng/mL upregulated the expression of PEDF of ORS cells, with IL-4 having the greater effect. PEDF 50 ng/mL decreased migration of ORS cells.
PEDF is expressed in human cutaneous appendages and may play a modulatory role in the physiology of ORS cells.