Conflict of interest: none declared.
Experimental dermatology ● Original article
Photodynamic therapy inhibits the formation of hypertrophic scars in rabbit ears by regulating metalloproteinases and tissue inhibitor of metalloproteinase-1
Version of Record online: 29 JAN 2014
© 2014 British Association of Dermatologists
Clinical and Experimental Dermatology
Volume 39, Issue 2, pages 196–201, March 2014
How to Cite
Wang, Q., Dong, Y., Geng, S., Su, H., Ge, W. and Zhen, C. (2014), Photodynamic therapy inhibits the formation of hypertrophic scars in rabbit ears by regulating metalloproteinases and tissue inhibitor of metalloproteinase-1. Clinical and Experimental Dermatology, 39: 196–201. doi: 10.1111/ced.12265
- Issue online: 13 FEB 2014
- Version of Record online: 29 JAN 2014
- Manuscript Accepted: 15 AUG 2013
- National Natural Science Foundation of China. Grant Number: 30901298
Hypertrophic scarring (HS) is a chronic skin condition, and inhibition of normal fibroblast ageing plays an important role in its pathogenesis. Photodynamic therapy (PDT) is known to inhibit synthesis of collagen proliferation in blood vessels and fibroblasts in scar tissue, with no significant adverse reactions reported.
To investigate the effect of PDT in the rabbit ear model of HS, and the specific mechanism of action of PDT.
We assessed the clinical and histopathological appearance of rabbit ears with HS with and without PDT. In addition, mRNA levels of matrix metalloproteinase (MMP)-2, MMP-3, MMP-9 and tissue inhibitor of metalloproteinase (TIMP)-1, and concentration of β-galactose were all measured to confirm cell senescence.
Our data indicate that PDT can accelerate fibroblast ageing by increasing the ratio of MMPs to TIMP, in addition to promoting degradation of collagen and extracellular matrix, thereby inhibiting HS formation. These effects lasted for up to 60 days, and induced no significant adverse local or systemic reactions. The efficacy of the treatment can be maximized by applying an appropriately high concentration of aminolaevulinic acid.
PDT can induce senescence in fibroblasts, and may constitute a useful treatment for pathological scarring.