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cei12013-sup-0001-si.tif536K

Fig. S1. Vitamin D (VD) serum level and immune status in patients subgrouped according to the human leucocyte antigen (HLA)-DR/DQ genotype. Type 1 diabetes (T1D) patients were subdivided into two groups: group A includes individuals with T1D high-risk genotypes HLA-DR3, HLA-DR4, HLA-DQ2 and HLA-DQ8. Group B includes patients with other HLA genotypes not associated with an elevated risk to develop T1D. The two groups were analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], mDCs (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0002-si.tif531K

Fig. S2. Vitamin D (VD) serum level and immune status in patients subgrouped according to the genotypes of the CYP24A1 rs2296237 polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the CYP24A1 rs2296237 polymorphism (CC, CG, GG) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], myeloid DCs (mDCs) (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0003-si.tif514K

Fig. S3. Vitamin D (VD) serum level and immune status in patients subgrouped according to the genotypes of the CYP24A1 rs927650 polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the CYP24A1 rs927650 polymorphism (TT, TC, CC) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], myeloid dendritic cells (mDCs) (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0004-si.tif516K

Fig. S4. Vitamin D (VD) serum level and immune status in patients subgrouped according to the genotypes of the CYP2R1 rs1074657 polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the CYP2R1 rs1074657 polymorphism (AA, AG, GG) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], myeloid dendritic cells (mDCs) (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0005-si.tif520K

Fig. S5. Vitamin D (VD) serum level and immune status in patients subgrouped according to the genotypes of the CYP2R1 rs12794714 polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the CYP2R1 rs12794714 polymorphism (AA, AG, GG) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], myeloid dendritic cells (mDCs) (Lin3, CD303, CD123lo, CD11chi, CD4+], regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0006-si.tif511K

Fig. S6. Vitamin D (VD) serum level and immune status in patients subgrouped according to the genotypes of the CYP27B1 rs10877012 polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the CYP27B1 rs10877012 polymorphism (AA, AC, CC) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], myeloid dendritic cells (mDCs) (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4- producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0007-si.tif511K

Fig. S7. Vitamin D (VD) serum level and immune status in patients subgrouped according to the genotypes of the CYP27B1 rs4646536 polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the CYP27B1 rs4646536 polymorphism (TT, TC, CC) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], myeloid dendritic cells (mDCs) (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0008-si.tif514K

Fig. S8. Vitamin D (VD) serum level and immune status in patients subgrouped according to the genotypes of the VD-binding protein (DBP) Hae III polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the DBP Hae III polymorphism (GG, GT, TT) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], myeloid dendritic cells (mDCs) (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0009-si.tif515K

Fig. S9. Vitamin D (VD) serum level and immune status in patients subgrouped according to the genotypes of the VD-binding protein (DBP) Sty I polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the DBP Sty I polymorphism (CC, AC, AA) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], myeloid dendritic cells (mDCs) (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0010-si.tif514K

Fig. S10. Vitamin D (VD) serum level and immune status in patients subgrouped according to the genotypes of the VDR Apa I polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the VDR Apa I polymorphism (AA, Aa, aa) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], myeloid dendritic cells (mDCs) (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0011-si.tif515K

Fig. S11. Vitamin D (VD) serum level and immune status in patients sub-grouped according to the genotypes of the VDR Bms I polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the VDR Bms I polymorphism (BB, Bb, bb) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], myeloid dendritic cells (mDCs) (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0012-si.tif504K

Fig. S12. VD serum level and immune status in patients subgrouped according to the genotypes of the VDR Fok I polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the VDR Fok I polymorphism (FF, Ff, ff) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], mDCs (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

cei12013-sup-0013-si.tif509K

Fig. S13. Vitamin D (VD) serum level and immune status in patients subgrouped according to the genotypes of the VDR Taq I polymorphism. Type 1 diabetes (T1D) patients were subdivided into the three different genotypes of the VDR Taq I polymorphism (TT, Tt, tt) and analysed for associations between the individual genotypes and (A) 25-VD and 1,25-VD serum level; (B) frequency of plasmacytoid dendritic cells (pDCs) [Linage cocktail 3 (Lin3), CD303+, CD123+, CD11c+], myeloid dendritic cells (mDCs) (Lin3, CD303, CD123lo, CD11chi, CD4+), regulatory T cells (Tregs) (CD4+, CD25+, forkhead box protein 3 (FoxP3)+; CD8+, CD25+, FoxP3+) and (C) interferon (IFN)-γ, interleukin (IL)-17 or IL-4-producing CD4 and CD8 T cells after overnight stimulation with phorbol myristate acetate (PMA)/ionomycin. Significant differences in the Mann–Whitney U-test are indicated (GraphPad Prism software).

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