Membrane-bound interleukin-21 and CD137 ligand induce functional human natural killer cells from peripheral blood mononuclear cells through STAT-3 activation
Article first published online: 10 MAR 2013
© 2012 British Society for Immunology
Clinical & Experimental Immunology
Volume 172, Issue 1, pages 104–112, April 2013
How to Cite
Wang, X., Lee, D. A., Wang, Y., Wang, L., Yao, Y., Lin, Z., Cheng, J. and Zhu, S. (2013), Membrane-bound interleukin-21 and CD137 ligand induce functional human natural killer cells from peripheral blood mononuclear cells through STAT-3 activation. Clinical & Experimental Immunology, 172: 104–112. doi: 10.1111/cei.12034
- Issue published online: 10 MAR 2013
- Article first published online: 10 MAR 2013
- Accepted manuscript online: 20 NOV 2012 10:45PM EST
- Manuscript Accepted: 12 NOV 2012
- National Natural Science Foundation. Grant Numbers: 81071858, 81273216
- Innovative Scientific Research Key Project of Shanghai Municipal Education Commission. Grant Number: 11ZZ105
- Leading Academic Discipline Project of Shanghai Municipal Education Commission. Grant Number: J50201
- Shanghai Key Laboratory of Tumor Microenvironment and Inflammation. Grant Number: 11DZ2260200
Fig. S1. Expression of CD137 ligand (CD137L) and membrane-bound interleukin (mbIL)-21 on the surface of engineered K562 cells. A: CD137L staining; B: mbIL-21 staining.
Fig. S2. Effects of JSI-124 on natural killer (NK) cells. A: Expression level and phosphorylation status of signal transducer and activator of transcription-3 (STAT-3) in primary natural killer (NK) cells after treatment with 20 ng/ml of interleukin (IL)-21 in the presence or absence of 0·1 μM JSI-124 for 24 h. B: NK cell viability was evaluated by fluorescence activated cell sorter (FACS) after different doses of JSI-124 treatment at different time-points. This was representative of three independent primary NK cells. Results were repeated with three independent expanded NK cells, and similar results were obtained.
Fig. S3. Signal transducer and activator of transcription-3 (STAT-3) inhibition impaired expression of natural killer (NK) cell receptors. NK cells were initially expanded for 2 weeks as described in Materials and methods, and then 1 × 107 expanded NK cells were continued to expand in the presence or absence of 0·1 μM JSI-124. Three days later, the expression of NK cell receptors was detected by fluorescence activated cell sorter (FACS). The percentage decrease was calculated by comparing the mean expression levels of JSI-124-treated cells to those of the untreated control cells; n = 4.
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