Fig. S1. Isolation of LPMC from ileocaecal resection specimens. (a) Lamina propria mononuclear cells (LPMCs) were isolated from the mid-point of the strictured segment, and the proximal ileal margin, of ileocaecal resection specimens. A representative specimen shows a stricture (arrows) at the ileocaecal valve region. The mucosa in the stricture appears cobblestoned and the wall is extremely thick. Asterisk indicates proximal margin and arrowhead points to the vermiform appendix. (b) The Crohn's Disease Histologic Index of Severity (CDHIS) was determined for haematoxylin and eosin (H&E)-stained slides prepared from the stricture and proximal margin and is shown as the mean (standard error of the mean, ***P = 0·0001 by t-test, n = 16.


Fig. S2. Granulocyte CD116 abundance in healthy controls and Crohn disease (CD) patients. Cell surface abundance of the granulocyte–macrophage colony-stimulating factor (GM-CSF) receptor CD116 was measured by flow cytometry in healthy controls (HC) and CD patients. (a) Representative granulocyte gate and results for CD116 for CD and HC are shown. Data are shown as the mean (standard error of the mean) for the frequency of granulocytes expressing CD116 and the CD116 mean fluorescence intensity (MFI) on granulocytes, n = 11 for healthy controls (HC) and n = 39 for CD.

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