Paediatric Crohn disease patients with stricturing behaviour exhibit ileal granulocyte–macrophage colony-stimulating factor (GM-CSF) autoantibody production and reduced neutrophil bacterial killing and GM-CSF bioactivity
Article first published online: 18 APR 2013
© 2013 British Society for Immunology
Clinical & Experimental Immunology
Volume 172, Issue 3, pages 455–465, June 2013
How to Cite
Jurickova, I., Collins, M. H., Chalk, C., Seese, A., Bezold, R., Lake, K., von Allmen, D., Frischer, J. S., Falcone, R. A., Trapnell, B. C. and Denson, L. A. (2013), Paediatric Crohn disease patients with stricturing behaviour exhibit ileal granulocyte–macrophage colony-stimulating factor (GM-CSF) autoantibody production and reduced neutrophil bacterial killing and GM-CSF bioactivity. Clinical & Experimental Immunology, 172: 455–465. doi: 10.1111/cei.12076
- Issue published online: 18 APR 2013
- Article first published online: 18 APR 2013
- Accepted manuscript online: 25 JAN 2013 02:49AM EST
- Manuscript Accepted: 18 JAN 2013
- Integrative Morphology and Flow Cytometry cores of the National Institutes of Health (NIH)-supported Cincinnati Children's Hospital Research Foundation Digestive Health Center. Grant Number: 1P30DK078392-01
- NIH. Grant Number: R01 DK078683
Fig. S1. Isolation of LPMC from ileocaecal resection specimens. (a) Lamina propria mononuclear cells (LPMCs) were isolated from the mid-point of the strictured segment, and the proximal ileal margin, of ileocaecal resection specimens. A representative specimen shows a stricture (arrows) at the ileocaecal valve region. The mucosa in the stricture appears cobblestoned and the wall is extremely thick. Asterisk indicates proximal margin and arrowhead points to the vermiform appendix. (b) The Crohn's Disease Histologic Index of Severity (CDHIS) was determined for haematoxylin and eosin (H&E)-stained slides prepared from the stricture and proximal margin and is shown as the mean (standard error of the mean, ***P = 0·0001 by t-test, n = 16.
Fig. S2. Granulocyte CD116 abundance in healthy controls and Crohn disease (CD) patients. Cell surface abundance of the granulocyte–macrophage colony-stimulating factor (GM-CSF) receptor CD116 was measured by flow cytometry in healthy controls (HC) and CD patients. (a) Representative granulocyte gate and results for CD116 for CD and HC are shown. Data are shown as the mean (standard error of the mean) for the frequency of granulocytes expressing CD116 and the CD116 mean fluorescence intensity (MFI) on granulocytes, n = 11 for healthy controls (HC) and n = 39 for CD.
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