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Fig. S1. Infliximab (IFX) suppresses Crohn's disease (CD) peripheral blood (PB) CD4+ T cell interleukin (IL)-21 and IL-17A mRNA expression and T helper type 17 (Th17) cell differentiation. Purified PB CD4+ T cells (1 × 106/ml) from CD patients and healthy controls were stimulated with anti-CD3 (5 μg/ml) and anti-CD28 (2 μg/ml) in the absence or presence of IFX (50 μg/ml) and IL-21R/Fc (20 μg/ml). After 48 h of culture the cultured PB CD4+ T cells were harvested, followed by extraction of total RNA, and levels of IL-21 (A), IL-17 (B) and Th17 cell transcription factor retinoic orphan receptor C (RORC) (C) were then analysed by quantitative real-time polymerase chain reaction (PCR). Gene expression was normalized to β-actin mRNA levels in each sample. Data represent average fold increases or decreases over baseline levels in healthy controls cultured in medium alone (defined arbitrarily as 1·0). *P < 0·005 versus healthy controls under the same stimulatory conditions. +P < 0·05 versus data from the same group cultured in medium alone. ΔP < 0·05 versus data from the same group stimulated with anti-CD3 and anti-CD28 monoclonal antibodies (mAbs). #P < 0·05 versus data from the same group stimulated with anti-CD3 and anti-CD28 mAbs in the presence of IFX.

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