These authors contributed equally to this study.
Anti-tumour necrosis factor therapy enhances mucosal healing through down-regulation of interleukin-21 expression and T helper type 17 cell infiltration in Crohn's disease
Version of Record online: 6 JUN 2013
© 2013 British Society for Immunology
Clinical & Experimental Immunology
Volume 173, Issue 1, pages 102–111, July 2013
How to Cite
Liu, C., Xia, X., Wu, W., Wu, R., Tang, M., Chen, T., Xu, F., Cong, Y., Xu, X. and Liu, Z. (2013), Anti-tumour necrosis factor therapy enhances mucosal healing through down-regulation of interleukin-21 expression and T helper type 17 cell infiltration in Crohn's disease. Clinical & Experimental Immunology, 173: 102–111. doi: 10.1111/cei.12084
- Issue online: 6 JUN 2013
- Version of Record online: 6 JUN 2013
- Accepted manuscript online: 4 FEB 2013 04:01AM EST
- Manuscript Accepted: 25 JAN 2013
- National Natural Science Foundation of China. Grant Numbers: 30971358, 81061120521
- Shanghai Science and Technology Commission. Grant Number: 12XD1404000
Fig. S1. Infliximab (IFX) suppresses Crohn's disease (CD) peripheral blood (PB) CD4+ T cell interleukin (IL)-21 and IL-17A mRNA expression and T helper type 17 (Th17) cell differentiation. Purified PB CD4+ T cells (1 × 106/ml) from CD patients and healthy controls were stimulated with anti-CD3 (5 μg/ml) and anti-CD28 (2 μg/ml) in the absence or presence of IFX (50 μg/ml) and IL-21R/Fc (20 μg/ml). After 48 h of culture the cultured PB CD4+ T cells were harvested, followed by extraction of total RNA, and levels of IL-21 (A), IL-17 (B) and Th17 cell transcription factor retinoic orphan receptor C (RORC) (C) were then analysed by quantitative real-time polymerase chain reaction (PCR). Gene expression was normalized to β-actin mRNA levels in each sample. Data represent average fold increases or decreases over baseline levels in healthy controls cultured in medium alone (defined arbitrarily as 1·0). *P < 0·005 versus healthy controls under the same stimulatory conditions. +P < 0·05 versus data from the same group cultured in medium alone. ΔP < 0·05 versus data from the same group stimulated with anti-CD3 and anti-CD28 monoclonal antibodies (mAbs). #P < 0·05 versus data from the same group stimulated with anti-CD3 and anti-CD28 mAbs in the presence of IFX.
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