2-Chlorodeoxyadenosine (cladribine) induces apoptosis in human monocyte-derived dendritic cells
Article first published online: 4 JUL 2013
© 2013 British Society for Immunology
Clinical & Experimental Immunology
Volume 173, Issue 2, pages 288–297, August 2013
How to Cite
Singh, V., Prajeeth, C. K., Gudi, V., Bénardais, K., Voss, E. V. and Stangel, M. (2013), 2-Chlorodeoxyadenosine (cladribine) induces apoptosis in human monocyte-derived dendritic cells. Clinical & Experimental Immunology, 173: 288–297. doi: 10.1111/cei.12109
- Issue published online: 4 JUL 2013
- Article first published online: 4 JUL 2013
- Accepted manuscript online: 27 MAR 2013 05:09AM EST
- Manuscript Accepted: 14 MAR 2013
- Merck Serono GmbH
Fig. S1. Expression of CD14, CD1a, CD11c, CD86 and human leucocyte antigen D-related (HLA-DR) on monocyte-derived dendritic cells (MoDC). Monocytes and ex-vivo differentiated immature (Im)DC and mature (m)DC were stained with respective antibodies and analysed by flow cytometry. Data were normalized to isotype controls and the percentage of positive cells and mean fluorescence intensity (MFI) are presented. Isotype controls (shaded) were included for each staining. Results are representative of three independent experiments.
Fig. S2. Effect of 2-chlorodeoxyadenosine (CdA) on phosphatidylserine (PS) exposure in blood dendritic cells (DC) and monocytes. Human peripheral blood mononuclear cells (PBMC) were isolated and left untreated or treated with CdA (1 and 10 μM) for 24 h. Blood DC and monocytes were identified by immunostaining and were assayed for apoptosis using annexin V-fluorescein isothiocyanate (FITC)/7-aminoactinomycin D (7-AAD) staining followed by flow cytometry. (a) Dot-plots representing gated CD141+ blood DC and CD14+ monocytes. (b) Dot-plots representing percentages of annexin V−/7-AAD−, annexin V+/7-AAD− and annexin V+/7-AAD+ blood DC and monocytes. Results are representative of three independent experiments.
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