Fig. S1. Expression of CD14, CD1a, CD11c, CD86 and human leucocyte antigen D-related (HLA-DR) on monocyte-derived dendritic cells (MoDC). Monocytes and ex-vivo differentiated immature (Im)DC and mature (m)DC were stained with respective antibodies and analysed by flow cytometry. Data were normalized to isotype controls and the percentage of positive cells and mean fluorescence intensity (MFI) are presented. Isotype controls (shaded) were included for each staining. Results are representative of three independent experiments.


Fig. S2. Effect of 2-chlorodeoxyadenosine (CdA) on phosphatidylserine (PS) exposure in blood dendritic cells (DC) and monocytes. Human peripheral blood mononuclear cells (PBMC) were isolated and left untreated or treated with CdA (1 and 10 μM) for 24 h. Blood DC and monocytes were identified by immunostaining and were assayed for apoptosis using annexin V-fluorescein isothiocyanate (FITC)/7-aminoactinomycin D (7-AAD) staining followed by flow cytometry. (a) Dot-plots representing gated CD141+ blood DC and CD14+ monocytes. (b) Dot-plots representing percentages of annexin V/7-AAD, annexin V+/7-AAD and annexin V+/7-AAD+ blood DC and monocytes. Results are representative of three independent experiments.

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